Corilagin对家兔动脉粥样硬化斑块及ox-LDL损伤的血管内皮细胞TLR4表达的影响  

Effects of Corilagin on the Expression of TLR4 in Atherosclerotic Plaques of Rabbits and ox-LDL-injured HUVEc

作　　者：刘韬 吴希军 朱万芸 陈建雯 阳敬 沈志强 刘光 柴文英[4] 陈鹏 LIU Tao;WU Xi-jun;ZHU Wan-yun;CHEN Jian-wen;YANG Jing;SHEN Zhi-qiang;LIU Guang;CHAI Wen-ying;CHEN Peng(Dept.of Pharmacology,Honghe College of Health Professions,Honghe Yunnan 661100;Wulian Rehabilitation Hospital,Wulian County,Rizhao Shandong 262300;Pharmaceutical College&Key Laboratory of Pharmacology for Natural Products of Yunnan Province,Kunming Medical University,Kunming Yunnan 650500;Dept.of Breast Surgery,The First Affiliated Hospital of Kunming Medical University,Kunming Yunnan 650032,China)

机构地区：[1]红河卫生职业学院药学系,云南红河661100 [2]五莲县康复医院,山东日照262300 [3]昆明医科大学药学院暨云南省天然药物药理重点实验室,云南昆明650500 [4]昆明医科大学第一附属医院乳腺外科,云南昆明650032

出　　处：《昆明医科大学学报》2020年第10期15-20,共6页Journal of Kunming Medical University

基　　金：云南省教育厅科学研究基金资助项目(2018JS692)。

摘　　要：目的研究Corilagin对家兔动脉粥样硬化斑块及ox-LDL损伤的血管内皮细胞TLR4蛋白及m RNA表达的影响,探索Corilagin防止动脉粥样硬化的初步机制。方法分别通过高脂饲料喂养家兔和体外培养人脐静脉内皮细胞,复制动脉粥样硬化疾病模型和ox-LDL损伤模型。利用Western Blot和Q-PCR方法分别观察Corilagin对家兔动脉粥样硬化斑块和人脐静脉内皮细胞TLR4蛋白和m RNA表达的影响。结果与正常对照组比较,模型对照组斑块中TLR4蛋白及其m RNA表达水平显著升高(P<0.05)。与模型组比较,Corilagin能够显著降低斑块及ox-LDL损伤的血管内皮细胞中TLR4蛋白及其m RNA的表达水平(P<0.05)。结论 Corilagin能明显下调家兔动脉粥样硬化斑块及ox-LDL损伤的血管内皮胞中TLR4蛋白和m RNA的表达。Objective To study the effects of corilagin on the protein and mRNA expression level of TLR4 in atherosclerotic plaques of rabbits and ox-LDL-injured human umbilical vein endothelial cells(HUVEC). Methods The rabbits and human umbilical vein endothelial cells(HUVEC) were used to duplicate the atherosclerotic model and ox-LDL-injured model. Western blotting assay and quantitative real-time PCR were used to test the effect of corilagin on mRNA and protein expression level of TLR4. Results Compared with the normal control group,the protein and mRNA expression level of TLR4 in the model control group was significantly increased(P < 0.05). The protein and mRNA expression level of TLR4 in the model control group was significantly decreased in corilagin-treated group as compared with the model group(P < 0.05). Conclusion Corilagin could significantly down-regulate protein and mRNA expression of TLR4 in atherosclerotic plaques of rabbits and ox-LDL-injured HUVEc.

关 键 词：CORILAGIN 动脉粥样硬化斑块 人脐静脉血管内皮细胞 TLR4 

分 类 号：R543.1[医药卫生—心血管疾病]