水稻灌浆期穗颈瘟发生对产量及茎节代谢产物的影响  

Effect of Panicle Neck Blast on Grain Yield and Stem Node Metabolites at the Rice Filling Stage

作　　者：潘争艳 刘博 蒋洪波 姚继攀 白元俊 徐正进[2] PAN ZhengYan;LIU Bo;JIANG HongBo;YAO JiPan;BAI YuanJun;XU ZhengJin(Liaoning Rice Research Institute,Shenyang 110101;College of Agriculture,Shenyang Agricultural University,Shenyang 110161)

机构地区：[1]辽宁省水稻研究所,沈阳110101 [2]沈阳农业大学农学院,沈阳110161

出　　处：《中国农业科学》2020年第20期4177-4188,共12页Scientia Agricultura Sinica

基　　金：国家重点研发计划(2018YFD0200200);辽宁省科学事业公益研究基金(GY-20180046);辽宁省博士后项目(178150)。

摘　　要：【目的】检测自然条件下稻瘟病感病品种茎节的代谢产物,从代谢物角度解析水稻品种感病和产量降低的原因,为粳稻品种感病机理研究和生态防控提供理论依据。【方法】以稻瘟病感病品种辽星1号和粳型恢复系C418为材料,灌浆期调查穗颈瘟发病率,同时采用气相色谱-质谱(GC-MS)非靶向代谢组学方法检测感病茎节和正常茎节的代谢产物,利用主成分分析(PCA)感病茎节与正常茎节之间代谢物组成,利用偏最小二乘法判别分析(PLS-DA)的变量权重值(VIP>1.0)进行两者之间差异代谢产物种类的筛选,比较两者糖类物质和氨基酸类物质的差异,从代谢物角度解析水稻品种产量降低的原因。成熟期进行结实率、千粒重、粒长、糙米率等产量和品质性状测定。【结果】辽星1号和C418穗颈瘟发病率分别为57.23%和82.94%。穗颈瘟显著降低一次枝梗整精米率、二次枝梗的结实率、千粒重、长宽比和整精米率,其他性状无显著差异。辽星1号和C418的茎节GC-MS检测均定性到358种代谢产物,感病茎节上调了73.46%和67.88%的代谢产物。PCA和PLS-DA分析可以将感病茎节和正常茎节的代谢产物清楚分开,辽星1号和C418的感病茎节和正常茎节之间具有明显不同的代谢产物表型,与正常茎节相比,辽星1号感病茎节诱导61种代谢产物相对含量显著变化,C418感病茎节诱导47种代谢产物相对含量显著变化,两个品种共有显著变化的代谢产物30种。其中,次生代谢产物香草酸、α-生育酚、脱氢抗坏血酸、三十烷醇、大豆苷元和N-乙酰-d-甘露糖胺的相对含量显著下调,辽星1号分别下调了87.03%、96.29%、77.95%、74.56%、86.48%和82.68%,C418分别下调了99.92%、99.68%、97.26%、86.67%、94.96%和76.74%。正常茎节中辽星1号这6种次生代谢产物的相对含量分别是C418的2.64、2.39、2.39、2.27、3.09和2.56倍;感病茎节上调了63.33%的糖类物质和68.42%的氨【Objective】The objective of this study is to detect the stem node metabolite of susceptible cultivars which suffered the rice blast under the natural condition, analyze the reason for rice cultivars susceptibility and yield reduction, and to provide a theoretical basis for the susceptible mechanism study of japonica rice and ecological control.【Method】With susceptible cultivar which suffered the rice blast Liaoxing1 and japonica rice restorer C418 as the materials, the yield and quality character including seed setting rate, 1000-grain weight, length, brown rice were measured at the maturation stage. The incidence of panicle neck blast was investigated at the filling stage, and GC-MS was carried out to analyze the metabolites of diseased stem nodes and normal stem nodes, the metabolite composition between diseased stem nodes and normal stem nodes was analyzed by principal components analyses(PCA), the variable weights(VIP>1.0) of partial least-squares discriminant analysis(PLS-DA) were used to screen the species of differential metabolites, the difference of carbohydrate and amino acid metabolites was compared, and the reasons for the decrease of rice yield were analyzed from the metabolites.【Result】The incidence of panicle neck blast of Liaoxing1 and C418 was 57.23% and 82.94%, respectively. The panicle neck blast significantly reduced the head rice rate of the primary branch, the seed set rate, 1000-grain weight, length-width ratio and head rice rate of the secondary branch, there was no significant difference in other characters. 358 metabolites were identified in the two cultivars, the metabolites of diseased stem node increased by 73.46% and 67.88%, respectively. The PCA and PLS-DA analysis could clearly identify the metabolites of diseased stem node and normal stem node. The metabolite phenotypes of Liaoxing 1 and C418 were obviously different between the diseased stem node and normal stem node. Compared with normal stem node, the relative content of 61 metabolites induced by Liaoxing 1 diseased

关 键 词：水稻 穗颈瘟 代谢产物 糖类 氨基酸 产量 

分 类 号：S511[农业科学—作物学]