响应面法优化针叶樱桃总黄酮的提取工艺及其抗氧化活性研究  

Optimization of extraction process of total flavonoids from Acerola cherry by response surface methodology and their antioxidant activities

作　　者：杨志[1] 李文义[1,2] 高云涛[1] 熊华斌[1] 陈毅坚[2] 杨慧娟 YANG Zhi;LI Wenyi;GAO Yuntao;XIONG Huabin;CHEN Yijian;YANG Huijuan(Joint Research Centre for International Cross-Border Ethnic Regions Biomass Clean Utilization in Yunnan, Yunnan Minzu University, Kunming 650500, China;Key Laboratory of Chemistry in Ethnic Medicinal Resources, State Ethnic Affairs Commission and Ministry of Education, Yunnan Minzu University, Kunming 650500, China;College of Standardization, China Jiliang University, Hangzhou 310018, China)

机构地区：[1]云南民族大学云南省跨境民族地区生物质资源清洁利用国际联合研究中心,云南昆明650500 [2]云南民族大学民族药资源化学国家民委-教育部重点实验室,云南昆明650500 [3]中国计量大学标准化学院,浙江杭州310018

出　　处：《浙江农业学报》2020年第10期1866-1872,共7页Acta Agriculturae Zhejiangensis

基　　金：云南省应用基础研究计划(2016FD057,2017FD117);浙江绍兴上虞标准化研究院项目。

摘　　要：为有效提取针叶樱桃中的总黄酮,在单因素实验基础上,采用响应面法研究樱桃破碎目数、乙醇体积分数、液料比对黄酮提取率的影响,并对提取的黄酮进行抗氧化活性评价。结果表明:最佳提取工艺为樱桃破碎目数63.13、乙醇体积分数49.71%、液料比29.85,此时黄酮提取率预测值为85.07 mg·100g-1,与验证实验值86.9 mg·100g-1相符,说明响应模型方法可靠;且提取率越高,提取液抗氧化活性越好,最佳提取工艺下的DPPH抗氧化当量为73.38。For effective extraction of total flavonoids from Acerola cherry,on the basis of single factor experiments,the effect of cherry crushing mesh number,ethanol concentration,and liquid-solid ratio were studied by response surface methodology.Meanwhile,the antioxidant activity of total flavonoids from Acerola cherry was assessed.The results showed that the optimum extraction conditions were:cherry crushing mesh number of 63.13,ethanol volume fraction of 49.71%,liquid-solid ratio of 29.85.Under the optimal conditions,flavonoids extraction yield was 86.9 mg·100g-1 and was closed to the predicted value of 85.07 mg·100g-1.The antioxidant activity of the extracts increased with the increase of extraction rate,and the DPPH antioxidant equivalent under the optimum extraction conditions was 73.38.

关 键 词：针叶樱桃 总黄酮 响应面法 抗氧化活性 

分 类 号：TS255.1[轻工技术与工程—农产品加工及贮藏工程]