结核分枝杆菌H37Rv株急性感染豚鼠模型的研究  

Study on guinea pig model of acute infection with Mycobacterium tuberculosis H37Rv strain

作　　者：康健 许承明 张薇 柏银兰 韩文东[3] 孙志平[3] 徐志凯 王丽梅 KANG Jian;XU Chengming;ZHANG Wei;BAIYinlan;HAN Wendong;SUN Zhiping;XU Zhikai;WANG Limei(Department of Microbiology and Pathogenic Biology,Air Force Military Medical University,Shaanxi Province,Xi′an710032,China;Department of Pediatric,Tangdu Hospital,Air Force Military Medical University,Shaanxi Province,Xi′an710032,China;the Third Level Biosafety Laborator,Fudan University,Shanghai200032,China)

机构地区：[1]空军军医大学微生物与病原生物学教研室,陕西西安710032 [2]空军军医大学唐都医院儿科,陕西西安710032 [3]上海复旦大学三级生物安全防护实验室,上海200032

出　　处：《中国医药导报》2020年第30期20-23,共4页China Medical Herald

基　　金：“艾滋病和病毒性肝炎等重大传染病防治”科技重大专项(2012ZX10003008-007)。

摘　　要：目的建立结核分枝杆菌(Mtb)H37Rv株急性感染豚鼠模型的方法。方法18只豚鼠按随机数字表法分为6组:空白剂量组、皮下高剂量组、皮下低剂量组、滴鼻高剂量组、滴鼻中剂量组和滴鼻低剂量组,每组3只。皮下高、低剂量组采用腹股沟皮下注射的方式分别感染2×10^6 CFU/200μL和1×10^6 CFU/200μL的Mtb H37Rv PBS悬液。滴鼻高、中、低剂量组采用滴鼻的方式分别感染5×10^5、5×104 CFU/50μL和5×10^3 CFU/50μL的Mtb H37Rv PBS悬液。感染4周后,检测各组豚鼠的肺脾荷菌数和组织病理学改变情况。结果感染4周后,皮下高剂量组的肺、脾Mtb荷菌数均高于皮下低剂量组,差异有统计学意义(P<0.05)。感染4周后,皮下高剂量组的肺Mtb荷菌数高于滴鼻高剂量组,差异有统计学意义(P<0.05)。感染4周后,皮下高、低剂量组和滴鼻高剂量组豚鼠的肺组织有明显充血,肺泡壁间隔增厚,滴鼻中、低剂量组豚鼠的肺组织仅有轻微充血。感染4周后,各组豚鼠脾组织病理变化不明显。结论采用腹股沟皮下感染的方式成功建立H37Rv株急性感染豚鼠模型,为结核病新型疫苗和药物的研究奠定了基础。Objective To establish a guinea pig model of acute infection with Mycobacterium tuberculosis(Mtb)H37Rv strain.Methods Eighteen guinea pigs were divided into six groups:blank-dose group,subcutaneous high-dose group,subcutaneous low-dose group,intranasal high-dose group,intranasal middle-dose group and intranasal low-dose group according to random number table method.There were three guinea pigs in each group.Subcutaneous high-dose group and subcutaneous low-dose group was infected with Mtb H37Rv PBS suspension of 2×10^6 CFU/200μL and 1×10^6 CFU/200μL respectively.Intranasal high-dose group,intranasal middle-dose group and intranasal low-dose group was infected with Mtb H37Rv PBS suspension of 5×10^5,5×104 CFU/50μL and 5×10^3 CFU/50μL respectively.After four weeks of infection,the number of bacteria and histopathological changes of lung and spleen in each group were detected.Results After four weeks of infection,the number of Mtb in lung and spleen of subcutaneous high-dose group was higher than that of subcutaneous low-dose group(P<0.05).After four weeks of infection,the number of Mtb in lung of subcutaneous high-dose group was higher than that of intranasal high-dose group(P<0.05).After four weeks of infection,the lung tissues of guinea pigs in subcutaneous high-dose group,subcutaneous low-dose group and intranasal high-dose group were obviously congested and the alveolar wall were thickened;the lung tissue of guinea pigs in intranasal middle-dose group and intranasal low-dose group had only slight hyperemia.After four weeks of infection,the pathological changes of spleen tissue in each group were not obvious.Conclusion The guinea pig model of acute infection with Mtb H37Rv is established successfully,which provides the research foundation for novel tuberculosis vaccines and new drugs.

关 键 词：结核分枝杆菌 豚鼠 动物模型 急性感染 

分 类 号：R-332[医药卫生]