重组枯草芽孢杆菌异源分泌表达麦芽三糖酶及其发酵优化  

Secreted Expression of Maltotriohydrolase in Bacillus subtilis and Fermentation Optimization

作　　者：楼志华 Lou Zhihua(Jiangsu OGO Biotechnology Co.,Ltd.,Changzhou 213300,China)

机构地区：[1]江苏省奥谷生物科技有限公司,江苏常州213300

出　　处：《现代食品》2020年第19期169-172,共4页Modern Food

摘　　要：为了实现麦芽三糖酶的商业化生产,本研究以枯草芽孢杆菌为宿主,构建了木糖诱导分泌表达载体,表达了来源于Thermobifida fusca NTU22的麦芽三糖酶基因,考察重组枯草芽孢杆菌表达麦芽三糖酶的能力。摇瓶验证了基因的功能表达之后,经过初步优化,最大发酵酶活为(108.4±2.84)U·mL-1。该研究为重组枯草芽孢杆菌发酵产麦芽三糖酶的工业化应用提供了参考。In order to realize the commercial production of maltotriohydrolase,in this study,Bacillus subtilis was used as the host to construct a xylose-inducing secretion expression vector and express the maltotriohydrolase gene derived from Thermobifida fusca NTU22.The ability of recombinant Bacillus subtilis to express maltotriase was investigated.After the shake flask verified the functional expression of the gene,after preliminary optimization,the maximum fermentation enzyme activity was(108.4±2.84)U·mL-1.This research provides a reference for the industrial application of recombinant maltotriase produced by Bacillus subtilis fermentation.

关 键 词：枯草芽孢杆菌 麦芽三糖酶 分泌表达 

分 类 号：TQ925.1[轻工技术与工程—发酵工程]