感觉神经植入联合电刺激修复胫骨骨缺损的实验研究  

Experimental study on sensory nerve implantation combined with electrical stimulation for repairing tibial bone defect

作　　者：姚玉英[1] 郭志利[1] 张宏[1] 贾丽丽[1] 金玉祥 范雅丽[1] YAO Yuying;GUO Zhili;ZHANG Hong;JIA Lili;JIN Yuxiang;FAN Yali(Shijiazhuang Medical College, Shijiazhuang 050000, Hebei,China)

机构地区：[1]石家庄医学高等专科学校,河北石家庄050000

出　　处：《现代中西医结合杂志》2020年第31期3465-3470,共6页Modern Journal of Integrated Traditional Chinese and Western Medicine

基　　金：石家庄市2020年度河北省医学科学研究重点课题计划项目(2020159)。

摘　　要：目的探讨感觉神经植入联合微电流电刺激对胫骨骨缺损的修复作用及其作用机制。方法将64只新西兰白兔随机分为模型组、植入神经组、电刺激组、联合组,每组16只。各组实验动物均经手术构建胫骨骨缺损模型,联合组于造模术中植入感觉神经,并于术后第1天进行电刺激;电刺激组于造模术后第1天进行电刺激;植入神经组于造模术中植入感觉神经;模型组不做其他干预。分别在术后第1,2,3,4周进行样本观察、X射线检查、组织形态学观察和骨密度以及神经肽物质降钙素基因相关肽(CGRP)表达水平测定。结果术后4周,联合组骨缺损区域产生大量骨组织,有新生骨皮质;植入神经组、电刺激组产生的骨痂比联合组少,能填充骨缺损部位;模型组仅有部分骨痂产生。术后2,3,4周,联合组新生骨组织面积、骨痂骨密度和骨痂中CGRP表达水平均显著高于其他组,且呈时间依赖性,差异均有统计学意义(P均<0.05)。结论感觉神经植入可有效增加具有分泌神经肽物质能力的神经组织数量,电刺激可有效促进神经分泌神经肽物质CGRP,二者联合可以大大加快骨缺损部位的愈合,并且效果优于单独感觉神经植入或电刺激。Objective It is to explore the repairing effect of sensory nerve implantation combined with microcurrent electrical stimulation on tibial bone defect and its mechanism.Methods 64 New Zealand white rabbits were randomly divided into model group,nerve implantation group,electrical stimulation group,and combination group,with 16 rabbits in each group.The experimental animals in each group were operated to construct tibial bone defect models.The combination group was implanted with sensory nerves during the modeling operation,and electrical stimulation was performed on the first day after the operation;the electrical stimulation group was given electrical stimulation on the first day after the modeling operation;the nerve implantation group was implanted with sensory nerves during modeling;the model group was not treated with other interventions except modeling.Sample observation,X-ray examination,histomorphology observation were performed and the bone density and expression levels of neuropeptide calcitonin gene-related peptide(CGRP)were measured at 1,2,3,and 4 weeks after surgery.Results In 4 weeks after surgery,a large amount of bone tissue and new bone cortex was found in the bone defect area of the combination group;the callus in the nerve implantation group and electrical stimulation group was less than that in the combination group,it could fill the bone defect;the model group had only partial bone scab occurred.In 2,3,and 4 weeks after surgery,the area of new bone tissue,callus bone density,and CGRP expression in callus of the combination group were significantly higher than those of the other groups,and they were time-dependent,the differences were statistically significant(all P<0.05).Conclusion Sensory nerve implantation can effectively increase the number of nerve tissues with the ability to secrete neuropeptides,and electrical stimulation can effectively promote neurosecretory neuropeptide CGRP,the combination of the treatment can greatly accelerate the healing of bone defects,and its effect is better th

关 键 词：胫骨骨折 骨缺损 电刺激 感觉神经 降钙素基因相关肽 

分 类 号：R-332[医药卫生]