茴香5种组织RNA提取方法比较  

Comparison of RNA Extraction Methods in Five Tissues of Fennel

作　　者：王晓敏[1,2,3,4] 王林[1] 王彩艳 潘兵青[1] 周雪荣 WANG Xiaomin;WANG Lin;WANG Caiyan;PAN Bingqing;ZHOU Xuerong(School of Agriculture,Ningxia University,Yinchuan 750021,China;Ningxia Facility Horticulture(Ningxia University)Technology Innovation Center,Yinchuan 750021,China;Key Laboratory of Modern Molecular Breeding for Dominant and Special Crops in Ningxia,Yinchuan 750021,China;Ningxia Modern Facility Horticulture Engineering Technology,Yinchuan 750021,China;Federal Scientific and Industrial Organization of Australia,Canberra 2600,Australia)

机构地区：[1]宁夏大学农学院,宁夏银川750021 [2]宁夏设施园艺(宁夏大学)技术创新中心,宁夏银川750021 [3]宁夏优势特色作物现代分子育种重点实验室,宁夏银川750021 [4]宁夏现代设施园艺工程技术研究中心,宁夏银川750021 [5]澳大利亚联邦科工组织,澳大利亚堪培拉2600

出　　处：《河南农业科学》2020年第11期54-60,共7页Journal of Henan Agricultural Sciences

基　　金：2012年度宁夏留学回国人员创新创业项目;2016年度教育部春晖计划项目。

摘　　要：为了筛选茴香各组织总RNA的最适提取方法,以宁夏海原地区的茴香为材料,比较了CTAB改良法、CTAB-水饱和酚法、Trizol改良法和OMEGA植物RNA提取试剂盒(简称试剂盒法)提取茴香根、茎、叶、花、种子总RNA的效果,分别采用核酸蛋白检测仪及琼脂糖凝胶电泳测定提取的各组织总RNA的浓度、纯度及完整性。结果表明,对于茴香根、茎、叶、花而言,试剂盒法提取的RNA质量最好,得率较高且耗时短;CTAB改良法提取的RNA质量较好,得率高,但耗时较长;Trizol改良法提取的RNA得率较高,但浓度及完整性差;CTAB-水饱和酚法提取的RNA得率及质量等各方面均较低。对于茴香种子而言,RNA提取效果最好的是CTAB改良法。In order to screen the optimal extraction method of total RNA from each tissue of fennel,in this study,the fennel from Haiyuan in Ningxia was used as the material,modified hexadecyl trimethyl ammonium bromide(CTAB)method,CTAB water saturated phenol method,modified Trizol method and OMEGA plant RNA extraction kit(the kit method for short)were compared in the effects of total RNA extraction from roots,stems,leaves,flowers and seeds of fennel.The concentration,purity and integrity of total RNA from each tissue of fennel were determinated by nucleic acid and protein detector as well as agarose gel electrophoresis.The results showed that for fennel roots,steams,leaves and flowers,the quality of RNA extracted by the kit method was the best,the yield was higher,and the extraction time was short.The quality of RNA extracted by the modified CTAB method was better,the yield was high,but the extraction time was longer.The yield of RNA extracted by the modified Trizol method was higher,but the concentration and integrity were poor.The yield and quality of RNA extracted by the CTAB water saturated phenol method were lower.For fennel seeds,the effect of RNA extraction by the modified CTAB was the best.

关 键 词：茴香 组织 RNA提取方法 提取效果 CTAB改良法 

分 类 号：S573.3[农业科学—作物学]