脐带间充质干细胞对严重切割伤致脑水肿的保护作用  

Protective role of umbilical cord mesenchymal stem cells on encephaledema after severe cut injury

作　　者：杨劼 马奎[1] 刘煜凡 张翠萍[1] 邹祖鹏 杨思明[1,2,3] 付小兵 Yang Jie;Ma Kui;Liu Yufan;Zhang Cuiping;Zou Zujteng;Yang Siming;Fu Xiaobing(Key Laboratory of Wound Repair and Regeneration of PLA,Wound Repair and Tissue Regeneration Research Center,Department of Medical Innovation Research,PLA General Hospital,Beijing 100048,China;Department of Dermatology,Fourth Medical Center,PLA General Hospital,Beijing 100048,China;PIA Medical College,Beijing 100853,China)

机构地区：[1]解放军总医院医学创新研究部创伤修复与组织再生研究中心,全军创伤修复与组织再生重点实验室,北京100048 [2]解放军总医院第四医学中心皮肤科,北京100048 [3]解放军医学院,北京100853

出　　处：《中华创伤杂志》2020年第10期932-937,共6页Chinese Journal of Trauma

基　　金：国家自然科学基金(81801909,81830064,81721092);北京市自然科学基金(7184245);军队“十三五”重大和学科建设项目(AWS17J005,2019-126)。

摘　　要：目的探讨严重切割伤导致脑水肿的程度以及脐带间充质干细胞(UC-MSCs)的保护作用。方法选取90只雌性C57L小鼠,使用手术刀片制备小鼠腹部严重切割伤模型。按随机数字表法分为对照组(20只)、切割伤组(20只)、白细胞介素-6抗体(IL-6-AB)伤前使用组(切割伤前18 h使用,15只)、IL-6-AB伤后使用组(切割伤后1 h使用,15只)和UC-MSCs组(20只)。检测脑组织含水量。ELISA法检测脑组织IL-6变化趋势,Western blot检测水通道蛋白(AQP-4)表达变化。结果大脑组织含水量检测证实,与对照组[(77.1±2.4)%]比较,切割伤组脑组织含水量显著增高[(81.5±1.8)%](P<0.05)。与切割伤组比较,UC-MSCs组[(76.8±2.4)%]及IL-6-AB伤前使用组[(76.2±2.9)%]脑组织含水量均显著降低(P<0.05),但IL-6-AB伤后使用组[(82.4±1.7)%]差异无统计学意义(P>0.05)。ELISA法检测发现,与对照组[(10.3±0.3)pg/ml]比较,切割伤组[(16.6±1.3)pg/ml]脑皮质IL-6表达水平升高(P<0.01);与切割伤组比较,UC-MSCs组[(10.7±0.6)pg/ml]及IL-6-AB伤前使用组[(10.1±0.4)pg/ml]脑皮质IL-6表达水平显著降低(P<0.01),而IL-6-AB伤后使用组[(14.9±1.2)pg/ml]差异无统计学意义(P>0.05)。Western blot检测发现,与对照组比较(1.0±0.1),切割伤组(2.4±0.5)脑皮质AQP-4蛋白表达水平显著增加(P<0.01);与切割伤组比较,UC-MSCs组(1.2±0.3)及IL-6-AB伤前使用组(1.0±0.1)脑皮质AQP-4蛋白表达水平显著降低(P均<0.01),但IL-6-AB伤后使用组(2.3±0.3)差异无统计学意义(P>0.05)。结论严重切割伤可升高小鼠脑内IL-6及AQP-4蛋白的表达水平,升高脑组织含水量,最终导致脑水肿的形成,而使用UC-MSCs可以抑制上述反应从而防治脑水肿的形成。Objective To observe the extent of brain edema caused by severe cut injury and the protective role of umbilical cord mesenchymal stem cells(UC-MSCs).Methods A total of 90 female C57L mice were selected and the models of severe cut injury were prepared with surgical blade.According to the random number table,the animals were divided into control group(20 mice),cut group(20 mice),interleukin 6 antibody(IL-6-AB)before cut group(administered IL-6-AB at 18 hours before cut,15 mice),IL-6-AB after cut group(administered IL-6-AB at 1 hour after cut,15 mice)and UC-MSCs group(20 mice).The extent of brain edema was detected,the level of IL-6 in brain tissue by ELISA method and the expression of aquaporin4(AQP-4)by Western blot assay.Results Brain water content test showed brain edema in cut group was(81.5±1.8)%,significantly higher than(77.1±2.4)%in control group(P<0.05).Compared with cut group,brain edema in UC-MSCs group[(76.8±2.4)%]and IL-6-AB before cut group[(76.2±2.9)%]were significantly decreased(P<0.05),while that in IL-6-AB after cut group[(82.4±1.7)%]was little decreased(P>0.05).ELISA showed the level of IL-6 in cut group was significantly increased in mouse brain[(16.6±1.3)pg/ml],when compared with control group[(10.3±0.3)pg/ml](P<0.01).Compared with cut group,the levels of IL-6 in UC-MSCs group[(10.7±0.6)pg/ml]and IL-6-AB before cut group[(10.1±0.4)pg/ml]were significantly decreased(P<0.01),while that in IL-6-AB after cut group[(14.9±1.2)pg/ml]was little decreased in mouse brain(P>0.05).Western blot assay showed that compared with control group(1.0±0.1),the expression of AQP-4 in cut group(2.4±0.5)was significantly increased in mouse brain(P<0.01).Compared with cut group,the expression of AQP-4 in UC-MSCs group(1.2±0.3)and IL-6-AB before cut group(1.0±0.1)were significantly decreased(P<0.01),while that in IL-6 AB after cut group(2.3±0.3)was little decreased in mouse brain(P>0.05).Conclusions Severe cut injury can increase brain water content and eventually lead to brain edema through upregulati

关 键 词：间充质干细胞 脑水肿 切割伤 

分 类 号：R742[医药卫生—神经病学与精神病学]