RNA结合蛋白LARP6影响胃癌浸润及预后  

RNA binding protein LARP6 affects invasion and prognosis of gastric cancer

作　　者：胡仁旺 龚建平[1] HU Renwang;GONG Jianping(Department of Gastrointestinal Surgery,Tongji Hospital,Tongji Medical College in Huazhong University of Science and Technology,Wuhan 430000,P.R.China)

机构地区：[1]华中科技大学同济医学院附属同济医院胃肠外科,武汉430000

出　　处：《中国普外基础与临床杂志》2020年第11期1408-1412,共5页Chinese Journal of Bases and Clinics In General Surgery

摘　　要：目的研究La相关蛋白6(LARP6)基因表达对胃癌患者预后的影响,并探讨其与免疫细胞浸润量的关系。方法通过癌症基因组图谱(TCGA)数据库,下载胃癌患者的临床生存信息及基因表达谱信息,分析LARP6基因表达和患者临床特征的关系,并采用Cox比例风险回归模型探索影响患者预后的危险因素,再利用Kaplan Meier plotter数据库验证。然后结合TIMER免疫数据库探索LARP6基因表达与免疫细胞浸润量的相关性,及其对胃癌患者预后的影响。结果胃癌患者中,LARP6基因的表达与病理分期、T分期和N分期有关(P<0.05),但与M分期和性别无关(P>0.05)。多因素Cox比例风险回归分析结果表明,年龄和LARP6基因表达均是预后的影响因素(P<0.05)。进一步采用Kaplan Meier plotter数据库进行验证,结果也表明,LAPR6基因高表达胃癌患者的总体生存(OS)和无进展生存(PFS)情况均差于LARP6基因低表达的患者(P<0.001)。根据TIMER数据库探索LARP6基因在胃癌患者中的表达水平与免疫细胞浸润量的相关性,结果表明,LARP6基因在胃癌患者中的表达水平与CD4+T细胞和巨噬细胞浸润量呈正相关关系(P<0.001),且高浸润量的巨噬细胞和高LARP6基因表达均是影响患者术后生存的危险因素(P<0.05)。结论巨噬细胞浸润量和LARP6基因表达是胃癌患者预后的影响因素,可以用来判断临床预后,LARP6基因可能是胃癌治疗的新靶点。Objective To explore the effect of La-related protein 6(LARP6)gene on the survival of postoperative patients with gastric cancer,and to explore its relationship with immune cell infiltration.M ethods The clinical survival information and gene expression information of gastric cancer patients were downloaded from The Cancer Genome Atlas(TCGA)database.The relationship between LARP6 gene expression and clinical characteristics of patients were analyzed.Cox proportion hazard regression model was used to find out the prognostic risk factors of gastric cancer patients,and then Kaplan-Meier plotter database was used to verify.Then the correlation between LARP6 gene expression and immunity was proved by Tumor IMmune Estimation Resource(TIMER)im mune database.Results In gastric cancer patients,the expression of LARP6 gene was related to pathological stage,T stage,and N stage(P<0.05),but not related to M stage and sex(P>0.05).Multivariate Cox proportion hazard regression analysis showed that age[Hl^=2.022,95%C7 was(1.287,3.176),P=0.002]and LARP6 gene expression[HR=l.l76y 95%C7 was(1.070,1.293),P<0.001]were prognostic factors.Further verified by Kaplan Meier plotter database,the results also showed that the overall survival(OS)and progression-free survival(PFS)of gastric cancer patients with high expression of LAPR6 gene were worse than those with low expression of LARP6 gene(P<0.001).TIMER database was used to explore the correlation between the expression level of LARP6 gene and im mune cell infiltration in patients with gastric cancer,and the results showed that the expression level of LARP6 gene in gastric cancer patients was positively correlated with the infiltration number of CD4+T cells and macrophage cell(P<0.001).Log-rank results showed that infiltration number of macrophage cell and LARP6 gene expression were risk factors for clinical prognosis of gastric cancer patients(P<0.05).Conclusions Macrophage cell andcell and LARP6 gene expression are risk factors for gastric cancer patients.LARP6 may be a new target for

关 键 词：胃癌 La相关蛋白6 RNA结合蛋白 预后 

分 类 号：R735.2[医药卫生—肿瘤]