不同临床病理特征非小细胞肺癌中miR-1269的表达及其对细胞周期的靶向调控作用  

Expression of miR-1269 in non-small cell lung cancer with different clinical pathological characteristics and its targeted regulation on cell cycle

作　　者：詹莉琼[1] 卢晏民[1] 崔杰[2] ZHAN Liqiong;LU Yanmin;CUI Jie(Respiratory and Critical Care Medicine,Shangqiu First People’s Hospital,Shangqiu,Henan 476000,China;Three tumors,Shangqiu First People’s Hospital,Shangqiu,Henan 476000,China)

机构地区：[1]商丘市第一人民医院呼吸与危重症医学科,河南商丘476000 [2]商丘市第一人民医院肿瘤三科,河南商丘476000

出　　处：《安徽医药》2020年第11期2248-2251,共4页Anhui Medical and Pharmaceutical Journal

摘　　要：目的研究不同临床病理特征非小细胞肺癌(NSCLC)中miR-1269的表达及其对细胞周期的靶向调控作用。方法收集2015年3月至2018年12月商丘市第一人民医院手术切除的NSCLC病灶和对应的癌旁病灶58例,检测miR-1269及细胞周期蛋白的表达量;培养肺癌A549细胞株,转染miR-1269的模拟物和抑制物后检测细胞周期蛋白的表达量。结果NSCLC病灶中miR-1269的表达量明显高于癌旁病灶(P<0.05)且低未分化、有淋巴结转移、有脉管浸润、有胸膜侵犯的NSCLC病灶中miR-1269的表达量明显高于高中分化、无淋巴结转移、无脉管浸润、无胸膜侵犯的NSCLC病灶(P<0.05);NSCLC病灶中Cy-clinD1、CDK4、CDK6的表达量明显高于癌旁病灶且与miR-1269呈正相关,p21Cip1、p27Kip1的表达量明显低于癌旁病灶且与miR-1269呈负相关(P<0.05)。miR1269模拟物组A549细胞中CyclinD1、CDK4、CDK6的表达量明显高于空白对照组、NC模拟物组,p21Cip1、p27Kip1的表达量明显低于空白对照组、NC模拟物组(P<0.05);miR-1269抑制物组A549细胞中CyclinD1、CDK4、CDK6的表达量明显低于空白对照组、NC抑制物组,p21Cip1、p27Kip1的表达量明显高于空白对照组、NC抑制物组(P<0.05)。结论miR-1269的高表达与NSCLC病理特征的变化有关且miR-1269能够靶向调节细胞周期蛋白的表达。Objective To investigate the expression of miR-1269 in non-small cell lung cancer(NSCLC)with different clinical pathological characteristics and its targeted regulation on cell cycle.Methods NSCLC lesions and corresponding pericancerous lesions were collected from March 2015 to December 2018 in Shangqiu First People’s Hospital,and the expression of miR-1269 and cell cycle protein was detected.The A549 cell line of lung cancer was cultured,and the expression of cell cycle protein was detected after transfection of mimic and inhibitor of miR-1269.Results The expression of miR-1269 in NSCLC lesions was significantly higher than that in adjacent lesions(P<0.05),and the expression of miR-1269 in NSCLC lesions with low-undifferentiated,lymph node metastasis,vascular invasion and pleural invasion were significantly higher than that in NSCLC lesions with high-middle differentiation,no lymph node metastasis,no vascular invasion and no pleural invasion(P<0.05).The expression of CyncliD1,CDK4,CDK6 in NSCLC lesions were significantly higher than those in adjacent lesions and positively correlated with miR-1269,the expression of p21Cip1 and p27Kip1 were significantly lower than those in adjacent lesions and negatively correlated with miR-1269(P<0.05).The expression of CyncliD1,CDK4,CDK6 in A549 cells of miR-1269 mimic group were significantly higher than those in blank control group and NC mimic group,the expression of p21Cip1 and p27Kip1 were significantly lower than those in blank control group and NC mimic group(P<0.05).The expression of CyncliD1,CDK4,CDK6 in A549 cells of miR-1269 inhibitor group were significantly lower than those in blank control group and NC inhibitor group,the expression of p21Cip1 and p27Kip1 were significantly higher than those in blank control group and NC inhibitor group(P<0.05).Conclusion The high expression of miR-1269 is related to the pathological changes of NSCLC,and miR-1269 can target the expression of cell cycle protein.

关 键 词：癌 非小细胞肺 细胞周期蛋白D1 细胞周期蛋白质依赖激酶类 miR-1269 病理特征 

分 类 号：R734.2[医药卫生—肿瘤]