高效液相色谱法测定罗汉参中的西瑞香素  

Determination of daphnoretin content in Luohanshen by high performance liquid chromatography

作　　者：李刚 林玉洁 张隆龙 赵阳[1] 李红霞[1] 孙姗姗[1] LI Gang;LIN Yu-Jie;ZHANG Long-Long;ZHAO Yang;LI Hong-Xia;SUN Shan-Shan(National Institute for Food and Drug Control,Beijing 100050,China;Beijing Tiantan Pharmaceutical Biotechnology Development Co.Ltd,Beijing 100050,China)

机构地区：[1]中国食品药品检定研究院,北京100050 [2]北京天坛药物生物技术开发公司,北京100050

出　　处：《食品安全质量检测学报》2020年第19期7081-7086,共6页Journal of Food Safety and Quality

摘　　要：目的建立高效液相色谱法测定罗汉参中西瑞香素含量的分析方法。方法样品经过乙醇溶液提取,超声30 min,水浴温度70℃,旋蒸定容。采用C18反向色谱柱(250 mm×4.6 mm, 5μm)分离,以甲醇-0.2%磷酸水溶液(56:44, V:V)为流动相等度洗脱,流速为1.0 mL/min,柱温为35℃,检测波长345 nm。结果西瑞香素浓度在1~20μg/mL内与色谱峰面积呈良好的线性关系,Y=1.2872X+0.0921,r2=0.9998。方法精密度为0.34%(n=6),样品中加标回收率的范围从99.9%~101.2%。测定结果的相对标准差为1.73%(n=6),检出限(S/N=3)为0.06μg/g。结论该方法灵敏度高,可快速准确对罗汉参中的西瑞香素进行检测。Objective To establish a method for the determination of daphnoretin in Luohanshen by high performance liquid chromatography. Methods The sample was extracted by ethanol solution, ultrasonic for 30 minutes, water bath temperature of 70 ℃, spin evaporation and constant volume. The separation was performed on reverse C18 column(250 mm×4.6 mm, 5 μm) with methanol-0.2% phosphoric acid aqueous solution(55:44, V:V) mobile phase. The flow rate was 1.0 mL/min. The column temperature was 35 ℃ and the detection wavelength was 345 nm. Results There was a good linear relationship between the peak area and the concentration of daphnoretin in the range of 1-20 μg/mL, Y=1.2872 X+0.0921, r2=0.9998. The precision of method was 0.34%(n=6). The recoveries of samples were from 99.9% to 101.2%. The relative standard deviation was 1.73%(n=6), and the limit of detection was 0.06 μg/g(S/N=3). Conclusion This method has high sensitivity and can be used for the rapid and accurate determination of daphnoretin in Luohanshen.

关 键 词：罗汉参 西瑞香素 高效液相色谱法 

分 类 号：R284.1[医药卫生—中药学] O657.72[医药卫生—中医学]