基于猪肝酯酶杂化“纳米花”即时比色法检测氰戊菊酯  

Porcineliver esterase hybrid nanoflowers for the point-of-care colorimetric detection of fenvalerate

作　　者：陆单 叶泰[1] 徐斐[1] 曹慧[1] 袁敏[1] 于劲松[1] 周禹丞 葛宇[2] 虞成华[2] 王震 LU Dan;YE Tai;XU Fei;CAO Hui;YUAN Min;YU Jinsong;ZHOU Yucheng;GE Yu;YU Chenghua;WANG Zhen(Shanghai Engineering Research Center for Food Rapid Detection,School of Medical Instrument and Food Engineering,University of Shanghai for Science and Technology,Shanghai 200093;Shanghai Institute of Quality Inspection and Technology Research National Food Quality Supervision and Inspection Center(Shanghai),Shanghai 200233;Shanghai Howsome Biotechnology Co.Ltd.,Shanghai 201108)

机构地区：[1]上海食品快速检测工程技术研究中心上海理工大学医疗器械与食品学院,上海200093 [2]上海市质量监督检验技术研究院国家食品质量监督检验中心(上海),上海200233 [3]上海皓信生物科技有限公司,上海201108

出　　处：《分析试验室》2020年第10期1155-1159,共5页Chinese Journal of Analysis Laboratory

基　　金：国家重点研发计划(2017YFC1600603);上海市科委科技支撑(农业重点科技攻关)项目(17391901500);上海市科技兴农项目(2019-02-08-00-12-F01144)资助。

摘　　要：建立了基于猪肝酯酶杂化"纳米花"的氰戊菊酯的比色检测方法。将猪肝酯酶杂化"纳米花"酶解与高锰酸钾氧化显色耦合,并通过琼脂糖凝胶将显色底物集成到离心管管盖上。利用扫描电镜、透射电镜和红外光谱对杂化"纳米花"进行了表征,并对检测条件进行了优化。在最优条件下,氰戊菊酯的线性范围为1.5~12μg/m L,检出限为0.26μg/m L,并选择黄浦江水样品进行加标回收实验,回收率为92.5%~108.7%。A colorimetric method for the detection of fenvalerate had been established based on the porcine liver esterase hybrid"nanoflowers".The enzymatic hydrolysis of porcine liver esterase"nanoflowers"was coupled with colorimetric detection by potassium permanganate oxidation.The chromogenic substrate was integrated into the centrifuge tube cover by agarose gel.The hybrid"nanoflowers"were characterized by scanning electron microscopy,transmission electron microscopy and infrared spectroscopy.And the detection conditions were also optimized.Under the optimal conditions,the linear range of fenvalerate was 1.5-12μg/m L with the detection limit of 0.26μg/m L.The practical performance of this method was evaluated by the spiked Huangpu River samples with recovery of 92.5%-108.7%.

关 键 词：杂化“纳米花” 即时检测 菊酯类农药 比色检测 

分 类 号：O657.3[理学—分析化学]