孵化期LED绿光照射对坝上长尾鸡胚胎期肌纤维发育的影响  

The Effects of LED Green Light Irradiation on the Embryonic Muscle Development of Bashang Long-tail Chicken in Incubation Period

作　　者：郝文文 张贝[1] 任一帆 孟婕 巩静 杨晴[1] 耿立英[1] 张传生[1] 李祥龙[1] HAO Wenwen;ZHANG Bei;REN Yifan;MENG Jie;GONG Jing;YANG Qing;GENG Liying;ZHANG Chuansheng;LI Xianglong(Hebei Key Laboratory of Specialty Animal Germplasm Resources Exploration and Innovation,Hebei Normal University of Science&Technology,Qinhuangdao Hebei,066600,China)

机构地区：[1]河北科技师范学院河北省特色动物种质资源挖掘与创新重点实验室,河北秦皇岛066600

出　　处：《河北科技师范学院学报》2020年第3期40-46,共7页Journal of Hebei Normal University of Science & Technology

基　　金：河北省教育厅重点项目(项目编号:ZD2018225);河北科技师范学院博士启动基金项目(项目编号:2018YB002);河北省现代农业产业技术体系蛋鸡产业创新团队岗位项目(项目编号:HBCT2013090206);河北省研究生创新资助项目(项目编号:CXZZSS2019125)共同资助。

摘　　要：为探讨单色绿光对坝上长尾鸡肉蛋兼用型品种鸡胚胎期肌肉发育的影响,选用160个的受精卵随机分成4组,试验组用波长为(525±10)nm的绿光照射12 h黑暗12 h的连续光照模式,光照度分别为弱光组30~80 lx,中光组90~140 lx,强光组150~200 lx;对照组始终保持黑暗。分别在12,14,16,18,20胚龄随机取3个种蛋,取胚胎的腿肌、胸肌制作石蜡切片,测量各胚龄腿肌和胸肌组织纤维的直径和横截面积。结果表明:20胚龄时,经中光组照射孵化的坝上长尾鸡腿肌肌纤维直径和横截面积、胸肌肌纤维直径显著高于其余3组(P<0.05),而经强光组照射孵化的坝上长尾鸡胸肌肌纤维直径和横截面积均显著小于黑暗组(P<0.05)。孵化期使用中光组(90~140 lx)绿光照射可以促进鸡胚胎期肌纤维发育,而强光组(150~200 lx)绿光照射对坝上长尾鸡胚胎期胸肌肌纤维发育有一定的抑制作用。160 fertilized eggs were randomly divided into four groupsto explore the effect of monochromatic green light on the embryonic muscle development of Bashang long-tail chicken,with the model of 12-hour green light with a wavelength of(525±10)nm and 12-hour darkness,in the groups of low light(30~80 lx),medium light(90~140 lx),and strong light(150~200 lx),the control groupswere always kept in dark.At 12,14,16,18,and 20 embryonic ages,3 eggs were randomly selected from each group.The leg muscles and breast muscles of the embryos were taken to make paraffin sections,and the diameter and cross-sectional area of the leg muscles and breast muscle tissue fibers of each embryonic age were measured.The results showed that at the age of 20 embryos,the diameter and cross-sectional area of the muscle fibers of the legs the breast of Bashang long-tail chicken incubated with medium light were significantly higher than those of the other three groups(P<0.05),while that of those incubated with strong light were significantly smaller than the dark group(P<0.05).The use of medium green light(90~140 lx)during the incubation period can promote the development of embryonic muscle fibers in chickens,and strong green light group(150~200 lx)had a certain inhibitory effect on the development of the breast muscle fibers in the embryonic stage of Bashang long-tail chicken.

关 键 词：坝上长尾鸡 胚胎 LED 绿光照射 肌肉发育 

分 类 号：S831.32[农业科学—畜牧学]