Prevalence,serotyping and drug susceptibility patterns of Escherichia coli isolates from kidney transplanted patients with urinary tract infections  

作　　者：Atefeh Najafi Khah Mojdeh Hakemi-Vala Shiva Samavat Mohammad Javad Nasiri 

机构地区：[1]Department of Microbiology,School of Medicine,Shahid Beheshti University of Medical Sciences,Tehran 1985717443,Iran [2]Department of Adult Nephrology,School of Medicine,Shahid Labbafinezhad Hospital,Shahid Beheshti University of Medical Sciences,Tehran 1666694516,Iran

出　　处：《World Journal of Biological Chemistry》2020年第3期112-118,共7页世界生物化学杂志（英文版）（电子版）

基　　金：Supported by Research Department of School of Medicine Shahid Beheshti University of Medical Sciences,No.17920,and accepted by the ethic committee,Code.IR.SBMU.MSP.REC.1398.349.

摘　　要：BACKGROUND Extended-spectrumβ-lactamase(ESBL)-producing Escherichia coli(E.coli)are among the main pathogens in urinary tract infections(UTIs)among kidney transplant patients(KTPs).AIM To estimate the prevalence of ESBL-producing E.coli in KTPs and to evaluate the most prevalent serotypes and antibacterial susceptibility patterns of isolated bacteria in Tehran,Iran.METHODS A total of 60 clinical isolates of uropathogenic E.coli were collected from 3 kidney transplant centers from April to May 2019.Antimicrobial susceptibility testing was performed by the disk diffusion method as recommended by the Clinical Laboratory and Standards Institute.The serotyping of E.coli isolates was performed by the slide agglutination method.The presence of blaTEM,blaSHV,and bla CTX-M genes was evaluated by polymerase chain reaction.RESULTS The frequency of ESBL-producing E.coli in KTPs was found to be 33.4%.All of the 60 E.coli isolates were found to be susceptible to doripenem(100%)and ertapenem(100%).High resistance rates to ampicillin(86%),cefotaxime(80%),and cefazolin(77%)were also documented.The most frequent serotypes were serotype I(50%),serotype II(15%),serotype III(25%),and serotype VI(10%).The gene most frequently found was blaTEM(55%),followed by blaCTX-M(51%)and blaSHV(41%).CONCLUSION Molecular analysis showed that blaTEM was the most common ESBL-encoding gene.The high resistance toβ-lactams antibiotics(i.e.,ampicillin,cefotaxime,and cefazolin)found in E.coli from KTPs with UTIs remains a serious clinical challenge.Further efforts to control ESBL-producing E.coli should include the careful use of all antibiotics as well as barrier precautions to reduce spread.

关 键 词：Kidney transplantation Urinary tract infection Drug resistance Escherichia coli SEROTYPING Β-LACTAMASE 

分 类 号：R699.2[医药卫生—泌尿科学] R446.5[医药卫生—外科学]