miRNA-133b在胃癌组织中的表达及其对MMP-9的影响  

Expression of miRNA-133b in gastric cancer tissue and its effect on MMP-9

作　　者：马国明[1] 左卫微 贾纯亮[1] 梁磊[1] 姚远[1] 李青科[1] 刘远廷[1] MA Guoming;ZUO Weiwei;JIA Chunliang;LIANG Lei;YAO Yuan;LI Qingke;LIU Yuanting(Department of Gastrointestinal Surgeryy,Tangshan Municipal People′s Hospital,Tangshan,Hebei 063000,China;Department of Obstetrics and Gynecology,Tangshan Municipal People′s Hospital,Tangshan,Hebei 063000,China)

机构地区：[1]河北省唐山市人民医院胃肠外科,063000 [2]河北省唐山市人民医院妇产科,063000

出　　处：《重庆医学》2020年第21期3624-3629,共6页Chongqing medicine

基　　金：2018年度河北省医学科学研究重点课题计划(20181228)。

摘　　要：目的探讨微小RNA-133b(miRNA-133b)在胃癌组织中的表达情况及其对基质金属蛋白酶9(MMP-9)表达的影响。方法选取2015年12月至2018年1月于该院胃肠外科住院行胃癌根治手术的患者78例,留取胃癌组织及癌旁组织(距肿瘤边界6 cm以上),实时荧光定量PCR(RT-qPCR)检测miRNA-133b与MMP-9 mRNA在两组标本中的表达水平,免疫组织化学(IHC)方法检测MMP-9在两组标本中的蛋白表达,应用Spearman相关性分析法对miRNA-133b和MMP-9 mRNA的表达量进行相关性分析,采用受试者工作特征(ROC)曲线分析胃癌组织中miRNA-133b表达水平对胃癌诊断的价值。结果与癌旁组织比较,胃癌组织中miRNA-133b的相对表达量明显下调,MMP-9 mRNA及蛋白表达水平明显升高,差异均有统计学意义(1.01±0.30 vs.0.45±0.19,1.06±0.44 vs.2.03±0.55,20.51%vs.65.38%,P<0.05);且胃癌组织中miRNA-133b表达水平与MMP-9 mRNA表达量呈明显负相关性(r=-0.667,P<0.05)。胃癌组织中miRNA-133b表达水平预测胃癌的ROC曲线下面积为0.942(95%CI:0.909~0.974,P<0.05),最优截断点为0.78,预测胃癌的灵敏度和特异度分别为91.02%和80.77%。结论胃癌组织中miRNA-133b表达降低可能通过靶向上调MMP-9的表达,促进胃癌细胞的浸润和转移,从而在胃癌发生、发展中起作用,且miRNA-133b表达水平对胃癌的诊断有重要价值。Objective To investigate the expression level of microRNA-133b(miRNA-133b)in gastric cancer tissues and its effect on the expression of matrix metalloproteinase-9(MMP-9).Methods A total of 78 cases of gastric cancer radical operation in the gastrointestinal surgery department of this hospital from December 2015 and January 2018 were selected.The gastric cancer tissue and paracancerous tissues(more than 6 cm from the tumor border)were obtained.RT-qPCR was used to detect the expression of miRNA-133b and MMP-9 mRNA in the samples,and the immunohistochemical(IHC)method was used to detect the protein expression of MMP-9 in the two groups of samples.The Spearman correlation analysis method was used to conduct the correlation analysis on the expression levels of miRNA-133b and MMP-9 mRNA.In addition,the ROC curve was used to analyze the value of miRNA-133b expression level for diagnosing gastric cancer.Results Compared with the paracancerous tissue,the expression level of miRNA-133b in the gastric cancer tissues was significantly down-regulated(P<0.05),and MMP-9 mRNA and protein expression levels were significantly increased,and the differences were statistically significant(1.01±0.30 vs.0.45±0.19,1.06±0.44 vs.2.03±0.55,20.51%vs.65.38%,P<0.05).Moreover the miRNA-133b expression level in the gastric cancer tissue had the significantly negative correlation with the expression level of MMP-9 mRNA(r=-0.667,P<0.05).The area under the ROC curve of the expression level of miRNA-133b in gastric cancer tissue for predicting gastric cancer was 0.942(95%CI:0.909-0.974),and best cut-off point was 0.78,the sensitivity for predicting gastric cancer was 91.02%,and the specificity was 80.77%.Conclusion The decrease of miRNA-133b expression in gastric cancer tissue may be involved in the occurrence and development of gastric cancer possibly by up-regulating the expression of MMP-9 to promote the infiltration and metastasis of gastric cancer cells,moreover the miRNA-133b expression level has an important value for diagnosing gastric

关 键 词：胃肿瘤 微RNA-133b 基质金属蛋白酶9 受试者工作特征曲线 曲线下面积 

分 类 号：R735.2[医药卫生—肿瘤]