TTK对膀胱癌细胞增殖、凋亡、侵袭及迁移的影响  

Effects of TTK on Proliferation,Apoptosis,Invasion and Migration of Bladder Cancer Cells

作　　者：刘锦裕[1] 谢建兵[1] 谢锦来[1] 晏湘山[1] 李永生[2] LIU Jinyu;XIE Jianbing;XIE Jinlai;YAN Xiangshan;LI Yongsheng(Department of Urology,the Affiliated Hospital of Putian University,Putian 351100,China;Union Clinical College of Fujian Medical University,Fuzhou 350001,China)

机构地区：[1]莆田学院附属医院泌尿外科,莆田351100 [2]福建医科大学附属协和医院泌尿外科,福州350001

出　　处：《中国细胞生物学学报》2020年第9期1517-1525,共9页Chinese Journal of Cell Biology

基　　金：福建省教育厅中青年教师教育科研项目(批准号:JAT190559)资助的课题。

摘　　要：该研究探讨了苏氨酸和酪氨酸激酶(threonine and tyrosine kinase,TTK)在膀胱癌中的表达情况及其在膀胱癌细胞增殖、凋亡、侵袭及迁移中的作用。采用qRT-PCR和免疫组织化学分别检测TTK mRNA和蛋白在癌旁正常组织、非肌层浸润性膀胱癌组织、肌层浸润性膀胱癌组织中的表达水平;将TTK过表达质粒、TTK敲除质粒借助脂质体分别稳定转染膀胱癌HT-1376细胞;用qRT-PCR和Western blot检测转染后TTK mRNA和蛋白的表达情况;应用CCK法和EdU方法检测细胞增殖活性;流式细胞术检测细胞周期分布及凋亡情况;transwell小室法检测细胞侵袭、迁移能力。结果显示:TTK mRNA和蛋白在癌旁正常组织、非肌层浸润性膀胱癌组织、肌层浸润性膀胱癌组织中的表达水平逐渐升高,3者之间差异有统计学意义(P<0.05);过表达TTK后,HT-1376细胞增殖能力增强,细胞凋亡减少,细胞体外侵袭和迁移能力增强;而敲除TTK后,HT-1376细胞生长受抑制,细胞周期阻滞在G0/G1期,细胞凋亡率增加,细胞体外侵袭和迁移能力减弱。该研究结果提示,TTK的表达与膀胱癌的发生、发展有关;TTK能促进膀胱癌HT-1376细胞的增殖、侵袭、迁移,并抑制细胞凋亡。The aim of this study was to explore the expression of TTK(threonine and tyrosine kinase)in bladder cancer tissues and the effect of TTK on the proliferation,apoptosis,invasion and migration of bladder cancer cells.qRT-PCR and immunohistochemistry were adopted to detect the expression of TTK mRNA and protein in paracancerous tissues,non-muscle-invasive bladder cancer tissues,and muscle-invasive bladder cancer tissues.TTK overexpression plasmid and TTK knockout plasmid were separately transfected into bladder cancer HT-1376 cells by lipofectamine.qRT-PCR and Western blot were used to detect the expression of TTK mRNA and protein after transfection;CCK and EdU assays were conducted to detect cell proliferation activity.Flow cytometry was used to detect cell cycle and apoptosis.The cell invasion and migration ability were determined by transwell assay.The results showed that the expression levels of TTK mRNA and protein in paracancerous tissues,non-muscle-invasive bladder cancer tissues,and muscle-invasive bladder cancer tissues gradually increased,and the differences among the three groups were statistically significant(P<0.05).After overexpressing TTK,the proliferation ability of HT-1376 cells was enhanced,the cell apoptosis rate was reduced,and the migration and invasion abilities of the cells were enhanced.After knocking out TTK,the proliferation of HT-1376 cells was reduced,the cell cycle was blocked at the G0/G1 phase,the cell apoptosis rate was increased,and the cell migration and invasion abilites were attenuated.The results of this study suggested that the expression of TTK is related to the progression of bladder cancer.TTK can promote the proliferation,invasion and migration of bladder cancer HT-1376 cells and induce cell apoptosis.

关 键 词：苏氨酸和酪氨酸激酶 膀胱癌 细胞增殖 凋亡 侵袭 

分 类 号：R737.14[医药卫生—肿瘤]