慢性乙型肝炎后肝硬化患者外周血T细胞亚群及促炎症亚群CD16^+单核细胞的变化及临床意义  

Changes and clinical significance of peripheral blood T cell subsets and pro-inflammatory subsets CD16^+ monocytes in patients with cirrhosis after chronic hepatitis

作　　者：孟玉丽[1] 王斌[1] 张红旭[1] MENG Yu-li;WANG Bin;ZHANG Hong-xu(Luohe Central Hospital,Luohe 462000,China)

机构地区：[1]河南省漯河市中心医院,河南漯河462000

出　　处：《实用医院临床杂志》2020年第6期23-26,共4页Practical Journal of Clinical Medicine

基　　金：河南省医学科技攻关计划项目(编号:201702335)。

摘　　要：目的分析慢性乙型肝炎后肝硬化患者外周血T细胞亚群及促炎症亚群CD16^+单核细胞的变化及临床意义。方法我院临床确诊的72例性乙型肝炎和70例乙型肝炎后肝硬化患者,再选取同时间段60例健康体检者为对照组,对各组人群的T细胞亚群及促炎症亚群CD16^+单核细胞的变化情况进行对比,分析各细胞因子在不同人群中的表达及临床意义。结果与对照组比较,乙型肝炎组和乙型肝炎后肝硬化组CD3^+、CD3^+CD4^+、CD3^+CD8^+、CD4^+/CD8^+计数明显偏低,其中乙型肝炎后肝硬化组最低(P<0.05);对照组CD14^+CD16^+亚群计数显著低于乙型肝炎组和乙型肝炎后肝硬化组,乙型肝炎后肝硬化组最高(P<0.05);乙型肝炎后肝硬化组CD14^++CD16^-亚群计数显著低于对照组和乙型肝炎组,对照组最高(P<0.05);慢性乙型肝炎后肝硬化患者促炎症亚群CD14^+CD16^+/CD14^+与肝炎病变程度呈正相关,外周血T细胞亚群以及CD14^+CD16-/CD14^+的表达与肝炎病变程度呈负相关(P<0.05)。结论外周血T细胞亚群、促炎症亚群CD16^+的表达与慢性乙型肝炎后肝硬化患者的肝炎病变程度具有相关性,可根据其变化程度对患者病变程度做有效的评估。Objective To investigate the changes and clinical significance of peripheral blood T cell subsets and pro-inflammatory subsets CD16^+monocytes in patients with cirrhosis after chronic hepatitis B.Methods Seventy-two patients with hepatitis B and 70 patients with cirrhosis after chronic hepatitis B in our hospital were selected,and 60 healthy patients who underwent physical examination at the same time were selected as controls.The changes of T cell subsets and pro-inflammatory subsets CD16^+monocytes in each group were compared,and the clinical significance of each cytokine in different people were analyzed.Results Compared with the control group,CD3^+,the counts of CD3^+CD4^+,CD3^+CD8^+and CD4^+/CD8^+were significantly lower in the hepatitis B group and the cirrhosis after chronic hepatitis B group,and the cirrhosis after chronic hepatitis B group was the lowest(P<0.05).The counts of CD14^+CD16^+subsets in the control group was significantly lower than those in the hepatitis B group and the cirrhosis after chronic hepatitis B group,and the cirrhosis after chronic hepatitis B group was the highest(P<0.05).The counts of CD14^+^+CD16-subsets in the cirrhosis after chronic hepatitis B group was significantly lower than that in the control group and the hepatitis B group,and the control group was the highest(P<0.05).Combined with histopathological examination and Spearman correlation analysis showed that the pro-inflammatory subsets CD14^+CD16^+/CD14^+was positively correlated with the degree of hepatitis lesions in patients with cirrhosis after chronic hepatitis B.The peripheral blood T cell subsets and the expression of CD14^++CD16^-/CD14^+were negatively correlated with the degree of hepatitis lesions(P<0.05).Conclusion The peripheral blood T cell subsets and pro-inflammatory subsets CD16^+is related to the degree of hepatitis lesions in patients with cirrhosis after chronic hepatitis B.The degree of the patient's disease can be effectively evaluated according to their changes.

关 键 词：慢性乙型肝炎 肝硬化 T细胞亚群 促炎症亚群CD16^+单核细胞 临床意义 

分 类 号：R575.1[医药卫生—消化系统] R512.6+2[医药卫生—内科学]