建立及超声评估血友病性关节炎动物模型  

Establishment and ultrasonic evaluation of hemophilic arthritis animal models

作　　者：刘昊 马菲[1] 张亚如 高扬 易梅 冯晓勤[2] 孙竞[3] 李颖嘉[1] LIU Hao;MA Fei;ZHANG Yaru;GAO Yang;YI Mei;FENG Xiaoqin;SUN Jing;LI Yingjia(Department of Ultrasound,Nanfang Hospital,Southern Medical University,Guangzhou 510515,China;Department of Pediatrics,Nanfang Hospital,Southern Medical University,Guangzhou 510515,China;Department of Hematology,Nanfang Hospital,Southern Medical University,Guangzhou 510515,China)

机构地区：[1]南方医科大学南方医院超声医学科,广东广州510515 [2]南方医科大学南方医院儿科,广东广州510515 [3]南方医科大学南方医院血液科,广东广州510515

出　　处：《中国医学影像技术》2020年第10期1441-1445,共5页Chinese Journal of Medical Imaging Technology

基　　金：南方医科大学南方医院2016-2017医疗技术院级研究课题(2016018)。

摘　　要：目的建立血友病性关节炎(HA)动物模型,以超声监测关节结构改变,并评价造模效果。方法将25只新西兰大白兔分为A、B组(各5只)及C组(15只)。对A、B组膝关节腔内注射兔动脉血液1 ml,A组每周3次,共36次,B组每周2次,共24次;C组注射1 ml右旋糖酐铁,按造模时间分为C1组(诱导4周)、C2组(诱导8周)、C3组(诱导12周),以超声观察关节腔内滑膜和软骨改变。造模结束后行病理学检查,观察关节软骨及滑膜病理变化。结果造模结束时,超声测得A组[(4.46±0.47)mm]及C组[(4.08±0.44)mm]滑膜厚度均大于B组[(2.43±0.39)mm](P均<0.05)。造模过程中,A、B组滑膜厚度及血流信号分级逐渐增加;C组造模初期滑膜厚度及血流信号逐渐增加,随造模时间延长,滑膜厚度略微变薄、血流信号逐渐减弱。造模结束时超声可见A组、C组股骨远端软骨损伤。各组光镜下均出现滑膜增生、炎细胞浸润、软骨破坏等类似HA病理改变,以A组及C1组较为显著。结论通过向关节腔内注射血液及右旋糖酐铁均可建立HA兔模型,其症状、超声及病理改变能模拟HA特征性表现。铁剂诱导型关节炎造模快速、简便,易于超声监测。Objective To establish rabbit models of hemophilic arthritis(HA)through injecting blood and iron dextran into articular cavity,and to monitor the changes of joint structures and evaluate the effect of modeling with ultrasound.Methods A total of 25 New Zealand white rabbits were divided into Group A,B(each n=5)and C(n=15).In group A and group B,1 ml rabbit arterial blood was injected into the articular cavity with a total of 36 times(3 times a week)in group A and 24 times(twice a week)in group B.In group C,1 ml of iron dextran was injected into the articular cavity of rabbits,and then the rabbits were averagely divided into 4 weeks group(C1 group),8 weeks group(C2 group)and 12 weeks group(C3 group).The changes of synovium and cartilage of the articular cavity were observed with ultrasound.Pathological examination was performed after modeling,and the pathological changes of articular cartilage and synovium were observed.Results After modeling,the synovium in group A([4.46±0.47]mm)and C([4.08±0.44]mm)measured with ultrasound were both thicker than in group B([2.43±0.39]mm,both P<0.05).In group A and B,the thickness and the grade of blood flow signal of synovial membrane increased gradually during modeling.In group C,the thickness and blood flow signal of synovial membrane increased gradually in the early stage of modeling but gradually thinned or weakened.At the end of modeling,damage of articular cartilage could be observed with ultrasound in group A and C.Synovial hyperplasia,infiltration of inflammatory cells and destruction of cartilage similar to HA were observed in each group under light microscope.The pathological changes in groups A and C1 were more significant than those in other groups.Conclusion Rabbit models of HA could be established through injecting blood or iron dextran into the articular cavity,both could reflect the characteristic manifestations of HA via symptoms,ultrasonic and pathological features.Iron induced arthritis models had advantages of short modeling period,simple operation and easy

关 键 词：关节炎 实验性 模型 动物 超声检查 

分 类 号：R-33[医药卫生] R445.1