基于拉曼镊子的发酵过程单细胞分析:应用与展望  

Single-cell Analysis of Microbial Fermentation Based on Raman Tweezers:Application and Prospect

作　　者：王桂文[1] 黄庶识[1] 陶站华[1] WANG Guiwen;HUANG Shushi;TAO Zhanhua(Guangxi Academy of Sciences,Nanning,Guangxi,530007,China)

机构地区：[1]广西科学院,广西南宁530007

出　　处：《广西科学院学报》2020年第3期260-271,共12页Journal of Guangxi Academy of Sciences

基　　金：国家自然科学基金项目(30760010,31060128);广西自然科学基金项目(2017GXNSFAA198029,2013GXNSFAA019043,2012GXNSFCA053001,桂科自0832022Z,桂科自0991079)资助。

摘　　要：微生物发酵是个复杂的过程,而细胞间的异质性对微生物发酵性能有重要的影响。通过单细胞分析了解微生物细胞的异质性,利于发酵环境的优化,从而减少细胞间的异质性,提高发酵性能。整合显微拉曼光谱和光学微操控的拉曼镊子是实时、无损、无须标记且快速灵敏的单细胞分析方法。本文重点回顾拉曼镊子在聚酯、类胡萝卜素、脂肪酸、生物乙醇以及重组蛋白等发酵生产过程中的应用,并分析其在应用上存在的不足。在克服自身不足、融合新的分析技术与数据处理方法后,拉曼镊子将是了解细胞异质性、认知复杂生物过程的有力手段。Microbial fermentation is a complex processing,and the heterogeneity between cells has a significant impact on the performance of microbial fermentation.Understanding the heterogeneity of microbial cells through single-cell analysis is beneficial to the optimization of the fermentation environment,thereby reducing the heterogeneity between cells and improving the performance of fermentation.Raman tweezers,a technique that integrates Raman micro-spectroscopy and optical micromanipulation,is a real-time,non-destructive,label-free,fast and sensitive single-cell analysis method.This article focuses on reviewing the application of Raman tweezers in the fermentation process of polyester,carotenoids,fatty acids,bio-ethanol and recombinant proteins,and analyzes the deficiencies in the application.After conquering its own shortcomings and integrating new analytical techniques and data processing methods,Raman tweezers is expected to be a more powerful method to reveal the cell heterogeneity and to recognize the complex biological processes.

关 键 词：微生物发酵 拉曼光谱 拉曼镊子 异质性 单细胞分析 

分 类 号：O657.37[理学—分析化学]