黄芪甲苷改善高糖受损内皮祖细胞生物学功能的实验研究  

Experimental Study of Astragaloside Ⅳ on Improving Biological Function of High Glucose Damaged Endothelial Progenitor Cells

作　　者：余亦程 熊武[2] 蔡昫 王禹萌[1] 肖慧 白雪 邹晓玲[1] YU Yicheng;XIONG Wu;CAI Xu;WANG Yumeng;XIAO Hui;BAI Xue;ZOU Xiaoling(Hunan University of Chinese Medicine,Changsha 410208,China;The First Hospital of Hunan University of Chinese Medicine,Changsha 410007,China)

机构地区：[1]湖南中医药大学,长沙410208 [2]湖南中医药大学第一附属医院,长沙410007

出　　处：《世界中医药》2020年第21期3244-3249,共6页World Chinese Medicine

基　　金：国家自然科学基金青年科学基金项目(81904217);湖南省卫生计生委科研计划课题项目(B20180739)。

摘　　要：目的:研究黄芪甲苷(Astragaloside Ⅳ,AS-Ⅳ)干预高糖受损人内皮祖细胞(Endothelial Progenitor Cells,EPCs)增殖的最佳浓度,并探讨AS-Ⅳ对高糖受损EPCs生物学功能的影响。方法:取足月新生儿脐带血分离、培养并鉴定EPCs,将鉴定成功的EPCs用30 mmol/L的葡萄糖预处理120 h后,随机分为实验组(HG+AS-Ⅳ组)和对照组(HG组),同时设置正常组(NC组)。用CCK8检测不同浓度AS-Ⅳ(0、25、50、100、200、400 mg/L)干预EPCs 24 h后的增殖情况,绘制增殖曲线,初步得出AS-Ⅳ干预高糖受损人EPCs增殖的最佳浓度。进而通过CCK-8增殖实验、黏附能力测定试验、细胞划痕试验及Matrigel体外成血管实验检测最佳浓度的AS-Ⅳ对高糖受损EPCs增殖、黏附、迁移和成管功能的影响。结果:AS-Ⅳ促高糖受损EPCs增殖的最佳浓度为100 mg/L;与对照组比较,100 mg/L AS-Ⅳ干预后的高糖受损EPCs增殖能力、黏附细胞数、细胞迁移率、体外成管数均明显增加,差异均有统计学意义(P1<0.05);同时检测实验组与正常组差异无统计学意义(P2>0.05)。结论:AS-Ⅳ可显著改善体外高糖受损的人EPCs的生物学功能,恢复其原有活力并具有介导血管新生的潜能。Objective:To study the optimal concentration of Astragaloside Ⅳ(AS-Ⅳ)on the proliferation of human endothelial progenitor cells(EPCs)impaired by high glucose,and to explore the biological effects of AS-Ⅳ on high glucose impaired EPCs.Methods:Full-term neonatal umbilical cord blood was isolated,cultured,and EPCs were identified.The identified EPCs were pretreated with 30 mmol/L glucose for 120 hours,and were randomly divided into an experimental group(HG+AS-Ⅳ group)and a control group(HG Group),while the normal group(NC group)was set.CCK8 was used to detect the proliferation of EPCs at different concentrations of AS-Ⅳ(0,25,50,100,200,400 mg/L)after 24 hours,and the proliferation curve was drawn to obtain the preliminary conclusion that AS-Ⅳ interfered with the proliferation of EPCs in patients with high glucose damage.Optimal concentration.Furthermore,the effects of the optimal concentration of AS-Ⅳ on the proliferation,adhesion,migration,and tube-forming function of high-glucose-damaged EPCs were tested by CCK-8 proliferation assay,adhesion ability test,cell scratch test,and Matrigel in vitro angiogenesis experiment.Results:The optimal concentration of AS-Ⅳ to promote the proliferation of high-glucose-damaged EPCs was 100 mg/L;compared with the control group,100 mg/L AS-Ⅳ-treated high-glucose-damaged EPCs had the proliferation capacity,the number of adherent cells,and cell migration The rate and the number of tube formation in vitro were significantly increased,and the differences were statistically significant(P1<0.05).At the same time,there was no significant difference between the experimental group and the normal group(P2>0.05).Conclusion:AS-Ⅳ can significantly improve the biological function of human EPCs with impaired high glucose in vitro,restore their original vitality and have the potential to mediate angiogenesis.

关 键 词：黄芪甲苷 内皮祖细胞 生物学功能 体外实验 

分 类 号：R285.5[医药卫生—中药学]