EB病毒衣壳抗原P18-23融合抗原的克隆表达及其在诊断中的应用  

Cloning and expression of Epstein-Barr virus capsid antigen P18-23 fusion antigen and its application in diagnosis

作　　者：陈咏梅[1] 张玲 王超男 胡纪文 张贺秋 冯晓燕 CHEN Yong-mei;ZHANG Ling;WANG Chao-nan;HU Ji-wen;ZHANG He-qiu;FENG Xiao-yan(Department of General Practice,Huabei Petroleum General Hospital,Renqiu,Hebei 062550,China;不详)

机构地区：[1]华北石油管理局总医院全科医学科,河北任丘062550 [2]东方海洋(北京)医学研究院,北京100071 [3]罗湖医院集团医学检验中心,广东深圳518001

出　　处：《中国卫生检验杂志》2020年第20期2451-2454,共4页Chinese Journal of Health Laboratory Technology

基　　金：山东省重点研发计划项目(2019JZZY011018)。

摘　　要：目的制备高活性EB病毒衣壳抗原P18-23融合抗原并初步评价该抗原在鼻咽癌诊断中的应用价值。方法利用分子生物学技术进行克隆表达获得纯化抗原,采用酶联免疫吸附试验初步抗原的检测性能。结果获得了高效原核表达的衣壳抗原P18-23融合抗原,建立了基于该抗原的IgA间接ELISA方法,检测灵敏度和特异度分别为89.77%和86.32%,抗VCA-IgA诊断NPC的AUC值为0.883。结论原核表达的衣壳抗原P18-23融合抗原具有良好的检测性能,利用该抗原建立的VCA-IgA间接ELISA检测方法可以很好地区分鼻咽癌患者和健康对照,适合用于NPC的鉴别诊断。Objective The Epstein-Barr virus capsid antigen P18-23 fusion antigen were prepared and its application value in the diagnosis of nasopharyngeal carcinoma was preliminarily evaluated.Methods The antigens were cloned and expressed using molecular biology technology,and their detection performance was preliminarily evaluated by enzyme-linked immunosor-bent assay(ELISA).Results The EB virus capsid antigen p18-23 fusion antigen was highly prokaryotic expressed in E.coil,and a VCA-IgA indirect ELISA method based on this antigen was established.The detection sensitivity and specificity were 89.77%and 86.32%respectively,and the AUC value of VCA-IgA was 0.883.Conclusion The recombinant virus capsid antigen P18-23 fusion antigen has good detection performance.The indirect ELISA detection method of VCA-IgA established using this antigen can be used to distinguish NPC patients from healthy controls,which is suitable for the differential diagnosis of NPC.

关 键 词：EB病毒 衣壳抗原 P18-23融合抗原 诊断 鼻咽癌 

分 类 号：R739.63[医药卫生—肿瘤]