20-HETE对肺腺癌A549细胞增殖、凋亡及迁移的影响  

The effect of 20-HETE on the proliferation,apoptosis and migration in lung adenocarcinoma A549 cells

作　　者：孔令慧 王悦[2] 贾茹 武晶晶[2] KONG Ling-hui;WANG Yue;JIA Ru;WU Jing-jing(Department of Biological Sciences;Department of Medical Genetics,School of Life Sciences,China Medical University,Shenyang 110122,China)

机构地区：[1]中国医科大学生命科学学院生物科学系 [2]中国医科大学生命科学学院医学遗传学教研室,辽宁沈阳110122

出　　处：《解剖科学进展》2020年第5期527-530,共4页Progress of Anatomical Sciences

基　　金：国家自然科学基金青年基金(81400709);辽宁省自然科学基金(2019-MS-381)。

摘　　要：目的探讨20-HETE对肺腺癌A549细胞增殖、凋亡和迁移能力的影响,初步探讨其分子机制。方法利用特异性20-HETE合成抑制剂HET0016处理A549细胞,CCK-8细胞增殖检测试剂盒和平板克隆实验分析20-HETE对A549细胞增殖及克隆形成能力的影响,流式细胞术检测20-HETE对A549细胞凋亡的影响,Transwell小室实验检测20-HETE对A549细胞迁移能力的影响;Western blot和葡萄糖检测试剂盒分析20-HETE对A549细胞葡萄糖代谢的影响。结果经HET0016处理后,A549细胞增殖、克隆形成和迁移能力显著下降,凋亡率明显上升,GLUT1蛋白表达下调,葡萄糖消耗下降。结论20-HETE促进A549细胞增殖、迁移,抑制细胞凋亡,与调控A549细胞葡萄糖代谢相关。Objective To investigate the effect and molecular mechanism of 20-HETE on proliferation,apoptosis and migration in lung adenocarcinoma A549 cells.Methods After treatment with HET0016,a specific synthetic inhibitor of 20-HETE,the effect of 20-HETE on A549 cell proliferation ability and clone formation ability was detected by CCK-8 cell proliferation assay kit and plate clone formation assay,the effect of 20-HETE on apoptosis was detected by flow cytometry,and the effect of 20-HETE on the cell migration ability was detected by Transwell.Western blot and glucose assay kit were employed to analyze the effect of 20-HETE on glucose metabolism.Results HET0016 significantly reduced A549 cell proliferation ability,clone formation ability and migration ability,raised apoptosis rate,decreased GLUT1 protein expression and glucose consumption.Conclusion 20-HETE promotes effectively A549 cells proliferation,clone formation and migration,reduced apoptosis,which is related to regulating the glucose metabolism of A549.

关 键 词：20-HETE 肺腺癌A549细胞 增殖 凋亡 迁移 葡萄糖代谢 

分 类 号：R734.2[医药卫生—肿瘤]