滇重楼内生真菌米曲霉中2个新的α-吡喃酮类化合物  

Two new α-pyronoids from endophytic fungus Aspergillus oryzae derived from Paris polyphylla var. yunnanensis

作　　者：于红梅 侯雨婷 祝丽君 陈彦君 陈毅坚[2] 董淼 周敏[2] 沈迪 陈名红[3] 叶艳青 YU Hong-mei;HOU Yu-ting;ZHU Li-jun;CHEN Yan-jun;CHEN Yi-jian;DONG Miao;ZHOU Min;SHEN Di;CHEN Ming-hong;YE Yan-qing(School of Chemistry and Environment,Yunnan Minzu University,Kunming 650500,China;National and Local Joint Engineering Research Center for Green Preparation Technology of Bio-based Materials,Kunming 650500,China;Key Laboratory of Chemistry in Ethnic Medicinal Resources,State Ethnic Affairs Commission&Ministry of Education,Kunming 650500,China)

机构地区：[1]云南民族大学化学与环境学院,云南昆明650500 [2]云南民族大学民族药资源化学国家民委教育部重点实验室,云南昆明650500 [3]云南民族大学生物基材料绿色制备技术国家地方联合工程研究中心,云南昆明650500

出　　处：《中草药》2020年第19期4891-4895,共5页Chinese Traditional and Herbal Drugs

基　　金：国家自然科学基金项目(31560099);国家自然科学基金项目(31860099);云南民族大学大学生创新创业训练项目;云南民族大学化学学科博士点培育项目(BSD-HX-2019-20)。

摘　　要：目的研究滇重楼内生真菌米曲霉Aspergillus oryzae次生代谢产物的化学成分及结构。方法将米曲霉采用液体发酵方式发酵,萃取后运用多种色谱方法进行分离纯化,并根据理化性质和波谱数据鉴定化合物的结构。结果从滇重楼内生真菌米曲霉发酵产物中分离鉴定了4个化合物,分别鉴定为4-羟基-6-[(2S,3S)-3-羟基丁烷-2-基]-3-甲基-2H-吡喃-2-酮(1)、(R)-4-羟基-6-(1-羟基-2-甲基丙基)-3-甲基-2H-吡喃-2-酮(2)、flufuran(3)、flufuran methyl ester(4)。结论化合物1、2为新的α-吡喃酮类化合物,命名为米曲霉α-吡喃酮A和米曲霉α-吡喃酮B。Objective To study the chemical composition and structure of the secondary metabolites of the endophytic fungus Aspergillus oryzae from Paris polyphylla var. yunnanensis. Methods A. oryzae was fermented by liquid fermentation. After extraction, it was separated and purified by various chromatography methods. The structure of the compounds was identified according to the physical and chemical properties and spectral data. Results Four compounds were isolated and their structures were identified as 4-hydroxy-6-[(2 S,3 S)-3-hydroxybutan-2-yl]-3-methyl-2 H-pyran-2-one(1),(R)-4-hydroxy-6-(1-hydroxy-2-methylpropyl)-3-methyl-2 H-pyran-2-one(2), flufuran(3) and flufuran methyl ester(4). Conclusion Compounds 1 and 2 are new α-pyronoids named asper-α-pyranone A and asper-α-pyranone B.

关 键 词：滇重楼 内生真菌 米曲霉 次生代谢产物 α-吡喃酮 米曲霉α-吡喃酮A 米曲霉α-吡喃酮B 

分 类 号：R284.2[医药卫生—中药学] R286.02[医药卫生—中医学]