磷酸酸蚀和不同磷酸酯单体预处理牙釉质表面的微观形态学观察和粗糙度评价  

Micromorphological observation and roughness evaluation of enamel surface pretreated by phosphoric acid etching and phosphoric acid ester monomers conditioning

作　　者：韩菲 陈晨[2] 杨家雪 石炜[2] 景双林[2] 谢海峰[1] HAN Fei;CHEN Chen;YANG Jiaxue;SHI Wei;JING Shuanglin;XIE Haifeng(Jiangsu Key Laboratory of Oral Diseases,Nanjing Medical University;Department of Prosthodontics,Affiliated Hospital of Stomatology,Nanjing Medical University,Nanjing 210029,China)

机构地区：[1]南京医科大学口腔疾病研究江苏省重点实验室南京医科大学附属口腔医院修复科,江苏南京210029 [2]南京医科大学口腔疾病研究江苏省重点实验室南京医科大学附属口腔医院牙体牙髓科,江苏南京210029

出　　处：《口腔医学》2020年第10期878-882,共5页Stomatology

基　　金：国家自然科学基金(81970927);江苏省自然科学基金(BK20191348);2019年江苏省青蓝工程优秀青年骨干教师;江苏高校优势学科建设工程资助项目(2018-87)。

摘　　要：目的探讨磷酸酸蚀不同时间和不同磷酸酯单体预处理对牙釉质表面粗糙度和微观形态的影响。方法切割一定规格的牙釉质试件并以钨钢车针抛光。分别使用35%磷酸酸蚀3、15、30、60 s,含有磷酸酯单体GPDM(glycerol phosphate dimethacrylate)的两步自酸蚀粘结剂Optibond Versa(OV)中的预处理剂和含有磷酸酯单体10-MDP(10-Methacryloyloxydecyl dihydrogen phosphate)的Tetric N Bond Universal(TNBU)进行表面预处理20 s,采用表面轮廓仪测量牙釉质表面粗糙度(Ra),与未处理组(Ctr)做对比,并通过扫描电镜(SEM)观察表面微观形态。结果35%磷酸酸蚀获得的表面粗糙度最高,其余依次是OV,TNBU和Ctr(P<0.05)。SEM观察显示,磷酸酸蚀3 s即可见釉柱轻度溶解,酸蚀15 s形成典型的鱼鳞状排列的蜂窝结构,随着酸蚀时间的延长,釉柱间隙愈发明显,釉柱结构出现塌陷。OV预处理剂处理过的牙釉质表面可观察到明显的釉柱边界。TNBU未见明显的釉柱蚀刻表现,划痕仍明显。结论磷酸酸蚀牙釉质时间以15 s为宜,经过磷酸酸蚀和OV预处理剂可以增加牙釉质表面粗糙度。OV预处理剂中的GPDM单体也可使牙釉质表面脱矿且脱矿深度大于TNBU中10-MDP。Objective To investigate the effects of different phosphoric acid etching time and conditioning with different phosphoric acid ester monomers on the surface roughness and micromorphology of enamel.Methods Enamel samples were polished and then pretreated with 35%phosphoric acid etching for 3,15,30 and 60 seconds,respectively.A two-step self-etch adhesive Optibond Versa(OV)primer containing phosphoric acid ester monomer GPDM(glycerol phosphate dimethacrylate)and Tetric N Bond Universal(TNBU)containing phosphoric acid ester monomer 10-MDP(10-Methacryloyloxydecyl dihydrogen phosphate)were used to pretreat enamel surface for 20 seconds.The enamel surface roughness(Ra)was tested by a profilometer and compared with the untreated group(Ctr),and the surface morphology was observed by scanning electron microscope(SEM).Results The surface roughness obtained by 35%phosphoric acid etching was the highest,followed by OV,TNBU and Ctr(P<0.05).SEM showed that the enamel-prism dissolved slightly after phosphoric acid etching for 3 seconds,and the typical fish scale-like honeycomb structure was formed after phosphoric acid etching for 15 seconds.With the increase of phosphoric acid etching time,the gaps between enamel-prisms became more obvious and the enamel-prism structure collapsed.The obvious enamel-prism contours could be observed on the enamel surface treated with OV primer.There was no obvious enamel-prism etching in TNBU,and the scratches were still clear.Conclusion Fifteen seconds of phosphoric acid etching on enamel is recommended.Enamel surface roughness can be increased by phosphoric acid etching and Optibond Versa.GPDM-containing primer can also achieve higher demineralization depth than that of 10-MDP containing adhesive.

关 键 词：牙釉质 磷酸酸蚀时间 磷酸酯单体 表面微观形态 表面粗糙度 

分 类 号：R783.1[医药卫生—口腔医学]