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机构地区:[1]College of Food Science and Technology,Henan University of Technology,Zhengzhou 450001,China [2]Henan Key Laboratory of Cereal and Oil Food Safety Inspection and Control Zhengzhou 450001,China
出 处:《Grain & Oil Science and Technology》2020年第3期117-125,共9页粮油科技(英文版)
基 金:supported by Program for Innovative Research Team(in Science&Technology)in University of Henan Province(No.20IRTSTHN023);supported by Key research and development and promotion special project of Science and Technology Department of Henan Province,China(No.192102310254)。
摘 要:The widespread contamination by mycotoxins,especially zearalenone and aflatoxins,in crops and their by-products has caused severe undesirable effects on human health and commercial trade.Researchers had screened out microorganisms from various media for degrading zearalenone and aflatoxins,and the results of a lot of studies showed that enzymes derived from microbial strains play a key role in degrading mycotoxins.Genetic engineering technology had been applied to improve the heterologously expressed degrading enzymes in several mature microbial hosts such as Escherichia coli and Pichia pastoris.The separated and purified recombinant enzyme had high activity in degrading mycotoxins in vitro.This review summarized the types of mycotoxins-degrading microorganisms and enzymes,and the progress on synthesis of heterologously expressed degrading enzymes by genetic engineering technology as well as related researches on improving the effect of degrading enzymes.We also prospected the future development in the study of using recombinant enzymes formed by genetic engineering technology to realize the simultaneous degradation of multiple mycotoxins in crops.
关 键 词:MYCOTOXIN Microbial degradation Genetic engineering techniques ZEARALENONE AFLATOXINS
分 类 号:TS201.3[轻工技术与工程—食品科学]
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