荧光各向异性信号放大策略研究进展  

Research Progress of Fluorescence Anisotropic Signal Amplification Strategy

作　　者：范垭玲 田智全[1,2] 庞代文[1] 张志凌[1] FAN Yaling;TIAN Zhiquan;PANG Daiwen;ZHANG Zhiling(College of Chemistry and Molecular Sciences,Wuhan University,Wuhan 430072;College of Science,Tibet University,Lhasa 850000)

机构地区：[1]武汉大学化学与分子科学学院,湖北武汉430072 [2]西藏大学理学院,西藏拉萨850000

出　　处：《分析科学学报》2020年第5期655-661,共7页Journal of Analytical Science

基　　金：国家自然科学基金(No.21775111)。

摘　　要：荧光各向异性分析法具有免分离、高通量、实时检测等优势,已广泛应用于生化分析领域。然而,由于待测物的体积或质量小,与探针结合不能产生明显的荧光各向异性信号变化,因此需采用一些荧光各向异性信号放大策略来解决这一问题。本文介绍了荧光各向异性法的检测基本原理,综述了荧光各向异性信号放大策略,并对荧光各向异性分析法进行了展望。In recent years,fluorescence anisotropy assays have been widely applied in diverse fields,especially in biochemical research,which provide excellent advantages,such as free separation or washing steps,high throughput,real-time analysis.However,due to the small volume or mass of the analyte,the combination with the probe only cause subtle fluorescence anisotropy signal changes,so it is important to employ some fluorescence anisotropy signal amplification strategies solve this problem.In this review,we initially introduce the general principle of fluorescence anisotropy assays.Next,we focus on fluorescence anisotropic signal amplification strategies including mass amplifying strategy,degree of free rotation of fluorescent probe and fluorescence lifetime.Finally,we end with an outlook on fluorescence anisotropy assays.

关 键 词：荧光各向异性 信号放大策略 免分离 实时检测 综述 

分 类 号：O657.39[理学—分析化学]