沉默长链非编码RNA HIF1A-AS2可抑制宫颈癌细胞的增殖、侵袭及迁移  

Silencing long non-coding RNA HIF1A-AS2 inhibits proliferation,invasion and migration of cervical cancer cells in vitro

作　　者：邓蓉 张福云[2] 雷福珍 刘文涓 刘丝荪[3] 王文华[2] DENG Rong;ZHANG Fuyun;LEI Fuzhen;LIU Wenjun;LIU Sisun;WANG Wenhua(Department of Gynecology,General Hospital of Pingxiang Mining Group CO.,Ltd.,Pingxiang 337003,China,China;Department of Gynecology,Pingxiang People's Hospital,Pingxiang 337000,China;Department of Obstetrics and Gynecology,First Affiliated Hospital of Nanchang University,Nanchang 330006,China)

机构地区：[1]萍乡矿业集团有限责任公司总医院妇科,江西萍乡337003 [2]萍乡市人民医院妇科,江西萍乡337000 [3]南昌大学第一附属医院妇产科,江西南昌330006

出　　处：《南方医科大学学报》2020年第11期1615-1621,共7页Journal of Southern Medical University

基　　金：江西省科技计划项目(20123BBG70189)。

摘　　要：目的探究长链非编码RNAHIF1A-AS2诱导宫颈癌细胞的上皮间质转换和肿瘤干细胞样特性的机制。方法设计3种可以沉默HIF1A-AS2的shRNA转染caski细胞株,选出干扰效果最好的shRNA进行后续研究;将caski细胞分为:Control组、shRNA-NC组和Sh-HIF1A-AS2组;检测各组细胞活力、侵袭能力、上皮间质转换及相关蛋白表达情况、肿瘤细胞成球情况和干细胞标记物情况;结果各组细胞活性差异无统计学意义(P>0.05);干扰结果确认使用HIF1A-AS2-shRNA1为shRNA;相比Control组,shRNA-NC组所有指标均无显著改变(P>0.05);相比shRNA-NC组,Sh-HIF1A-AS2组单位面积侵袭细胞数目、Vimrntin和N-cadherin、细胞成球数目、成球细胞直径、ABCG2阳性细胞率、Nanog、OCT4、SOX2蛋白和mRNA相对表达均显著降低,E-cadherin相对表达显著升高(P<0.05)。结论沉默长链非编码RNAHIF1A-AS2可以抑制宫颈癌细胞的增殖、侵袭及迁移能力。Objective To explore the inhibitory effects of silencing long non-coding RNA(LncRNA)HIF1A-AS2 on epithelialmesenchymal transition(EMT)and tumor stem cell-like phenotype in cervical cancer cells.Methods We designed 3 shRNA constructs for silencing HIF1A-AS2 in CaSki cells,and the shRNA with the strongest interference effect was selected for subsequent experiment.CaSki cells were transfected with shRNA-NC or Sh-HIF1A-AS2,and the changes in cell viability,invasion ability,EMT,expressions of EMT-related proteins,formation of cell spheres and expressions of stem cell markers were detected.Results Transfection with shRNA-NC and Sh-HIF1A-AS2 did not significantly affected the viability of CaSki cells(P>0.05).Compared with the cells transfected with shRNA-NC,the cells transfected with Sh-HIF1A-AS2 showed significantly reduced invasion ability,expressions of vimentin N-cadherin,and cell sphere formation ability.HIF1A-AS2 silencing obviously lowered the rate of ABCG2-positive cells,significantly reduced the mRNA and protein expressions of Nanog,OCT4,and SOX2,and strongly enhanced the expression of E-cadherin in CaSki cells(P<0.05).Conclusion Silencing HIF1A-AS2 can inhibit proliferation,invasion and migration of cervical cancer cells in vitro.

关 键 词：宫颈癌 长链非编码RNAHIF1A-AS2 上皮间质转换 肿瘤干细胞 

分 类 号：R737.33[医药卫生—肿瘤]