自动红细胞比容校正全血CRP测试结果特定蛋白分析仪的系统设计  

Design for the Automated Protein Analyzer Using Hematocrit to Calibrate the Results of Whole Blood CRP

作　　者：陈月玲[1] 王永强[2] 郑继波 陈兰兰[4] CHEN Yueling;WANG Yongqiang;ZHENG Jibo;CHEN Lanlan(Department ofVertigo,Shandong Provincial Hospital Affiliated to Shandong University,Jinan Shandong 250022,China;Department ofEquipment Management,The No.4 People’s Hospital of Jinan,Jinan Shandong 250031,China;Department of Information Network,Shandong Provincial Third Hospital,Jinan Shandong 250021,China;Department of Clinical Laboratory Medicine,Shandong Provincial Hospital Affiliated to Shandong University,Jinan Shandong 250021,China)

机构地区：[1]山东大学附属山东省耳鼻喉医院眩晕科,山东济南250022 [2]济南市第四人民医院器材设备管理科,山东济南250031 [3]山东省立第三医院信息网络部临床医学检验部,山东济南250021 [4]山东大学附属山东省立医院临床医学检验部,山东济南250021

出　　处：《中国医疗设备》2020年第11期80-84,共5页China Medical Devices

摘　　要：目的现有的自动化仪器,均采用固定的红细胞比容(Hematocrit,HCT)值换算血清C-反应蛋白(C-Reactive Protein,CRP)值。当HCT为非正常值时,需要查询血球仪实测的HCT值手工换算,本文设计一种可测试HCT的特定蛋白仪,实现自动校正功能。方法采用散射光对全血样本进行特定蛋白浓度及HCT的测定:验证全血CRP浓度值与HCT的线性关系,散射光强度与HCT的线性关系;使用散射法对特定蛋白浓度及HCT进行测定;利用HCT对全血CRP的校正。结果全血CRP浓度值与HCT成线性关系,R2=0.9819。散射光强度与HCT成线性关系,R2=0.9672;本文使用HCT为38%的样本所测得的CRP值为真值(49.15 mg/L),计算与经校正的CRP浓度值的相对偏差,均小于15%,在标准规定范围内。结论该设备实现的自动HCT校正CRP值,能有效将非正常HCT样品的CRP值校正到实际值。Objective The existing automated instruments all use fixed hematocrit(HCT)value to convert the serum C-reactive protein(CRP)value.The serum CRP value needs manual reckoning if the hematocrit is not in normal.In this paper,we designed a kind of device which can test the hematocrit with automatic correction function.Methods The specific protein concentration and hematocrit were measured by scattering light:the linear relationship between CRP concentration and hematocrit,and the linear relationship between scattering light intensity and hematocrit were verified;specific protein concentration and hematocrit were measured by scattering method;correct the CRP results using whole blood hematocrit.Results There was a linear relationship between the whole blood CRP concentration and hematocrit,R2=0.9819,as well as between the scattering light intensity and hematocrit,R2=0.9672.This paper takes the sample whose hematocrit is 38%as the true value(49.15mg/L).All the relative deviations are less than 15%which was within the standard range.Conclusion The analyzer uses hematocrit to adjust the CRP value.The results show that this procedure is effectively.

关 键 词：红细胞比容 C-反应蛋白 特定蛋白 分析仪 ARM 

分 类 号：R197.39[医药卫生—卫生事业管理]