微生态制剂在早期结直肠癌根治术后的作用  

Effect of microecological agents on early colorectal cancer after radical resection

作　　者：张英丽[1] 陈倩倩 刘颖 ZHANG Yingli;CHEN Qianqian;LIU Ying(Department of Oncology,Nanyang Central Hospital,Nanyang,Henan 473000,China;不详)

机构地区：[1]南阳市中心医院肿瘤科,河南南阳473000

出　　处：《中国微生态学杂志》2020年第10期1167-1172,共6页Chinese Journal of Microecology

摘　　要：目的探讨微生态制剂在早期结直肠癌(CRC)根治术后的作用,并为此类患者提供一种术后康复支持疗法。方法选取2017年6月-2019年6月于本院接受早期CRC根治术术后97例患者作为研究对象,采用随机数字表分为观察组50例和对照组47例。两组术后均接受常规治疗,观察组在对照组基础上接受双歧杆菌三联活菌胶囊治疗,比较两组治疗前后细胞免疫指标、肠道微生态复杂度及属水平相对丰度、肠道菌群OTU数量、肠道黏膜屏障功能。结果治疗后观察组NK细胞、CD3^+T、CD4^+T、CD4^+/CD8^+T均提高(P<0.05),观察组CD8^+T治疗前后相近(P>0.05);治疗后对照组上述指标与治疗前差异均无统计学意义(P>0.05),观察组NK细胞、CD3^+T、CD4^+T、CD4^+/CD8^+T均高于对照组(P<0.05);治疗后观察组Chao1指数、Shannon指数、肠道菌群OTU数均升高(P<0.05),对照组Chao1指数、Shannon指数、肠道菌群OTU数量治疗前后差异均无统计学意义(P>0.05);治疗后观察组Chao1指数、Shannon指数、肠道菌群OTU数量均高于对照组(P<0.05);治疗后观察组大肠杆菌、肠球菌、葡萄球菌属水平相对丰度均下降(P<0.05),对照组均升高(P<0.05),且观察组均低于对照组(P<0.05);治疗后观察组乳杆菌、双歧杆菌、梭菌、类杆菌、链球菌属水平相对丰度均升高(P<0.05),对照组均下降(P<0.05),且观察组均高于对照组(P<0.05);治疗后观察组血浆D-乳酸、DAO、I-FABP、内毒素水平分别为(6.94±1.23)mg/L、(3.45±0.65)U/L、(43.95±7.99)μg/L、(4.48±0.85)U/L,均较治疗前下降(P<0.05),对照组各项指标水平分别为(15.59±2.79)mg/L、(7.74±1.35)U/L、(74.21±13.82)μg/L、(8.68±1.65)U/L,均较治疗前升高(P<0.05),治疗后观察组血浆D-乳酸、DAO、I-FABP、内毒素水平均降低(P<0.05)。结论微生态制剂可有效改善早期CRC患者根治术后的细胞免疫水平,有效纠正早期CRC术后患者肠道菌群失调,增强早期CRC根治术后患者肠黏膜屏Objective To observe the effect of probiotics on early colorectal cancer(CRC)patients after radical resection,and provide a postoperative rehabilitation support therapy for such patients.Methods Ninety-seven patients who received early CRC radical surgery in our hospital from June 2017 to June 2019 were enrolled and divided into observation group(50 patients)or control group(47 patients).Both groups received conventional treatment after surgery,while the observation group received additionally triple viable Bifidobacterium capsules.Cellular immune indexes,microecological complexity and relative abundance at genus level,number of OTU,and intestinal mucosal barrier function before and after treatment were compared between groups.Results The levels of NK cells,CD3^+T,CD4^+T and CD4^+/CD8^+T increased in the observation group after treatment(all P<0.05),all of which were higher than those in the control group(P<0.05).The level of CD8^+T in the observation group was similar to that before treatment(P>0.05).The indexes in the control group after treatment were all similar to those before treatment(P>0.05).Chao1 index,Shannon index and number of OTU in the observation group increased after treatment(P<0.05),which were higher than those in the control group(P<0.05).In the control group,Chao1 index,Shannon index and numbers of OTU were similar to those before treatment(P>0.05).After treatment,the relative abundances of Escherichia coli,Enterococcus and Staphylococcus at genus level in the observation group decreased(P<0.05),while those in the control group increased(P<0.05),with those in the observation group being lower than those in the control group(P<0.05).After treatment,the relative abundances of Lactobacillus,Bifidobacteria,Clostridium,Bacteroides and Streptococcus at genus level in the observation group increased(P<0.05),while those in the control group decreased(P<0.05),with those in the observation group being higher than those in the control group(P<0.05).After treatment,the plasma levels of D-lactic acid,DAO,I

关 键 词：微生态制剂 结直肠癌 细胞免疫 肠道微生态 黏膜屏障 

分 类 号：R735.3[医药卫生—肿瘤]