不同营养水平饲粮对妊娠环江香猪体液游离氨基酸浓度的影响  

Effects of different dietary nutrient-levels on free amino acid concentration in body fluid of pregnant Huanjiang mini-pigs

作　　者：赵越 赵蕾 姬玉娇[1] 耿梅梅[1] 孔祥峰[1,3] ZHAO Yue;ZHAO Lei;JI Yujiao;GENG Meimei;KONG Xiangfeng(Key Laboratory for Agro-Ecological Processes in Subtropical Region/Hunan Provincial Key Laboratory of Animal NutritionPhysiology and Metabolism Process,Institute of Subtropical Agriculture,Chinese Academy of Sciences,Changsha 410125,China;College of Animal Science and Technology,Hunan Agricultural University,Changsha 410128,China;Research Center ofMini-Pig,Huanjiang Observation and Research Station for Karst Ecosystems,Chinese Academy of Sciences,Huanjiang 547100,China)

机构地区：[1]中国科学院亚热带农业生态研究所亚热带农业生态过程重点实验室/动物营养生理与代谢过程湖南省重点实验室,湖南长沙410125 [2]湖南农业大学动物科学技术学院,湖南长沙410128 [3]中国科学院环江喀斯特生态试验站香猪研究中心,广西环江547100

出　　处：《南京农业大学学报》2020年第6期1072-1078,共7页Journal of Nanjing Agricultural University

基　　金：国家自然科学基金项目(31772613,31572421);中国科学院STS区域重点项目(KFJ-STS-QYZD-052);广西科技基地和人才专项(桂科AD17195043);湖南创新型省份建设专项(2019RS3022)。

摘　　要：[目的]本试验旨在研究不同营养水平饲粮对妊娠母猪体液游离氨基酸浓度的影响。[方法]选用妊娠环江香猪48头,随机分为NRC饲粮组和地方猪饲粮组,每组8个重复(栏),每重复3头。分别于妊娠45和75 d,从每重复随机取1头母猪,前腔静脉采血,分离血浆;屠宰后收集每窝中最大体质量、中间体质量和最小体质量胎猪(对应)的羊水和尿囊液,测定游离氨基酸浓度。[结果]与地方猪饲粮组相比,饲喂NRC饲粮可显著提高妊娠45 d母猪血浆Leu、Lys、Gly和总氨基酸(TAA)浓度,显著提高最大体质量胎猪羊水中Leu浓度,以及妊娠75 d母猪血浆必需氨基酸(EAA)浓度(P<0.05);显著降低妊娠45 d母猪血浆Met浓度,妊娠75 d母猪非必需氨基酸和TAA浓度,以及妊娠45和75 d最小体质量胎猪羊水中Val和Lys浓度(P<0.05)。与妊娠45 d相比,妊娠75 d母猪2个饲粮组各体质量胎猪羊水中Met、Lys和Ile浓度以及尿囊液中Cys浓度均显著下降(P<0.05),尿囊液中Leu和Tau浓度显著升高(P<0.05),地方猪饲粮组各体质量胎猪羊水中Tau浓度均显著下降(P<0.05)。[结论]与饲喂地方猪饲粮相比,饲喂NRC饲粮可增加母猪对氨基酸的摄入,有利于胎猪的生长发育。[Objectives]The present study was conducted to investigate the effects of different dietary nutrient-levels on free amino acid concentration in plasma,amniotic fluid,and allantoic fluid of pregnant sows.[Methods]Forty-eight pregnant Huanjiang mini-pigs were randomly assigned into NRC diet group and local-pig diet group,with eight replicates(pens)per group and three pigs per replicate.At 45 and 75 d of pregnancy,blood samples from one sow per replicate were collected by cranial vena cava to separate plasma.The amniotic fluid and allantoic fluid of fetuses with maximal body weight(BW),middle BW,and minimal BW per litter were collected for determining free amino acid concentration.[Results]Compared with the local-pig diet,NRC diet significantly increased(P<0.05)the plasma concentration of Leu,Lys,Gly,and total amino acids(TAA),and the Leu concentration in amniotic fluid of fetuses with maximal BW at 45 d of pregnancy,as well as the plasma concentration of essential amino acid(EAA)at 75 d of pregnancy;while significantly decreased(P<0.05)the plasma Met concentration at 45 d of pregnancy,the plasma concentration of nonessential amino acid and TAA at 75 d of pregnancy,as well as the concentration of Val and Lys in amniotic fluid of fetuses with minimal BW at 45 and 75 d of pregnancy.The concentration of Met,Lys,and Ile in amniotic fluid and Cys in allantoic fluid of fetuses with the same standard BW in both diet groups significantly decreased(P<0.05)at 75 d of pregnancy,as well as the taurine(Tau)concentration in amniotic fluid of fetuses with various standard BW in local-pig diet group,while the concentration of Leu and Tau in allantoic fluid significantly increased(P<0.05),when compared with 45 d of pregnancy.[Conclusions]These findings suggested that,compared with the local-pig diet,NRC diet could result in the growth and development of fetuses by increasing the intake of amino acids of Huanjiang mini-sows.

关 键 词：妊娠环江香猪 营养水平 血浆 羊水 尿囊液 游离氨基酸 

分 类 号：S828[农业科学—畜牧学]