氟化氨银添加谷胱甘肽后的细胞毒性及对牙齿着色的影响  

Effects of ammonium fluoride with glutathione on cytotoxicity and tooth discoloration

作　　者：吴融慧 董青 王琳 Wu Ronghui;Dong Qing;Wang Lin(School of Stomatology,North China University of Technology,Tangshan 063200)

机构地区：[1]华北理工大学口腔学院,唐山063200

出　　处：《口腔材料器械杂志》2020年第4期223-228,共6页Chinese Journal of Dental Materials and Devices

摘　　要：目的研究氟化氨银(silver diamine fluoride,SDF)添加谷胱甘肽(Glutathione,GSH)涂于牙齿后的细胞毒性及对牙本质着色的影响。方法45个离体牙分成SDF组、SDF+20%GSH和空白组3组。每组15个样本,将暴露牙本质面涂药后,用电脑比色仪采集涂药后1 h、1 d、1周和1个月4个不同时间点的牙本质颜色值;CCK-8试剂盒评定SDF加20%GSH后对L929细胞毒性影响;将3组不同药物配制成适宜浓度培养L929细胞,最后用酶标仪检测吸光度值;倒置相差显微镜观察细胞生长形态。结果电脑比色仪结果显示SDF组颜色改变最明显,且随时间延长着色明显加深,SDF+GSH组在各时间段可以明显降低牙釉质的着色程度,空白组各时间均未见牙齿颜色的改变;细胞毒性实验显示:3组均没有细胞毒性作用。结论SDF添加GSH涂于牙齿后可以降低牙本质着色,同时对L929细胞生长没有影响。Objective The aim of this study was to investigate the effects of 38%ammonium fluoride on the tooth discoloration and the cytotoxicity of L929 after adding glutathione.Methods 45 isolated teeth were divided into three groups(15 samples in each group):(1)SDF group(2)SDF with 20%GSH(3)blank group.After coating the exposed dentin surface,the dentin color values at 1h,1 day,1 week and 1 month after application were collected by a computer colorimeter.CCK-8 kit was used to evaluate the cell viability of L929 cells after exposed to SDF plus 20%GSH.At 3,5,and 7 days,the absorbance value was measured with a microplate reader and the growth curve of L929 cells was drawn;the cell growth morphology was observed by inverted phase contrast microscope.Results The results of computer colorimeter showed that the color change of SDF group was the most obvious and the color was changed in a time-dependent manner.The coloration of teeth in the SDF+GSH group was reduced at all time periods,while no tooth color change was observed in the blank group at all times.Cytotoxicity experiments showed that none of the three groups had cytotoxic effects.Inverted phase contrast microscopy showed that the cells of three groups grew well.Conclusion Silver diamine fluoride could reduce enamal discoloration after adding glutathione.The addition of glutathione into silver diaminefluoride had no effect on the growth of L929 cells.

关 键 词：氟化氨银 谷胱甘肽 牙齿着色 细胞毒性 

分 类 号：R788.1[医药卫生—口腔医学]