转基因稻谷Bt蛋白降解及长期取食对印度谷螟体内3种抗氧化酶活性的影响  

Degradation of Bt Protein in Stored Transgenic Rice and its Effect on the Activity of Three Protective Enzymes in Indian Meal Moth,Plodia interpunctella(Hübner)

作　　者：王康旭 吴学友[1] 陶冶心 陈二虎 唐培安[1] WANG Kang-xu;WU Xue-you;TAO Ye-xin;CHEN Er-hu;TANG Pei-an(Collaborative Innovation Center for Modern Grain Circulation and Safety,College of Food Science and Engineering,Nanjing University of Finance and Economics,Nanjing,Jiangsu 210023,China)

机构地区：[1]南京财经大学食品科学与工程学院,江苏高校粮油质量安全控制及深加工重点实验室,江苏南京210023

出　　处：《粮油食品科技》2020年第6期111-117,共7页Science and Technology of Cereals,Oils and Foods

基　　金：粮食公益性行业科研专项(201413007-02,201513002-05-03);国家重点研发计划(2016YFD0401004-4,2017YFD0401003-5);江苏省六大人才高峰高层次人才项目(NY-057);江苏高校优势学科建设工程资助项目。

摘　　要：转Cry1Ab/Cry1Ac基因稻谷(华恢1号)可以用于储粮害虫印度谷螟的防治,前期研究表明在其短期取食含有Bt毒蛋白的稻谷后,其体内三种主要的抗氧化酶过氧化物酶(peroxidase,POD)、过氧化氢酶(catalase,CAT)和超氧化物歧化酶(superoxide dismutase,SOD)的活力会发生明显变化。聚焦储藏期间的特性开展研究,结果发现,转基因稻谷稻壳和糙米中Bt蛋白含量分别为5.534μg/g和1.003μg/g,在25℃下经过270 d,其降解率分别为67.3%和27.7%。利用酶活力测定方法研究长期取食转Bt稻谷所制大米粉对印度谷螟幼虫体内上述三种酶活性的影响,结果表明,在含有不同比例的转Bt大米粉饲喂印度谷螟12 d及连续饲养5个月后,其体内的SOD活力均受到了显著抑制。但是对于POD而言,在长期取食不同含量的转Bt大米粉后,印度谷螟体内POD活性与对照相比产生了显著提高。同时,在印度谷螟幼虫长期取食转Bt大米粉后,其体内CAT活力随着含Bt大米粉比例的提高而显著增强。另外,使用转基因大米粉连续饲养5个月的印度谷螟种群体内的保护酶活力同喂食12d后的酶活变化趋势一致。结果说明在长期取食含有Bt毒素的大米粉后,印度谷螟幼虫体内三种保护酶的增降趋势存在差异,这表明印度谷螟体内三种保护酶可能存在不同的调节机制来应对Bt毒素的胁迫,这对于转Bt作物的毒理和相关抗性机理研究提供了理论依据。Transgenic cry1Ab/cry1Ac rice(Huahui 1)is able to control the stored pest Plodia interpunctella.Our previous study confirmed that the enzymatic activities of three major antioxidases,including POD,CAT,and SOD,could be significantly changed after short-term stressed with these Bt containing rice.This study focused on the characteristics of rice during storage,the results showed that the Bt protein content in rice husk and brown rice was 5.534μg/g and 1.003μg/g respectively,and after 270 days at 25℃,the degradation rate was 67.3%and 27.7%,respectively.Moreover,the enzymatic activities of three antioxidases in P.interpunctella long-term fed with transgenic cry1Ab/cry1Ac rice powder would be addressed by enzymatic detection assays.Our data showed that the SOD activities could be significantly inhibited by the Bt containing rice after fed with Bt-rice powder for 12 days or 5 months,However,the enzymatic activities of POD and CAT significantly increase with the concentrations of Bt toxins.In addition,the change trend of protective enzyme activity in the Indian Meal Moth fed with transgenic rice flour for 5 months was consistent with that after 12 days of feeding.Our researches find that there are differences in the trend of increase and decrease of the three major protective enzymes after fed with Bt containing rice powder for a long time,which indicates that the expression mechanisms of the three protective enzymes might be different.Our results provide a theoretical basis for the study of toxicology and related resistance mechanism of transgenic Bt crops.

关 键 词：转基因稻谷 BT蛋白 晶体蛋白 印度谷螟 保护酶 过氧化物酶 氧化氢酶 超氧化物歧化酶 

分 类 号：TS201.4[轻工技术与工程—食品科学]