过氧化物酶1在胃癌细胞上皮间充质转化过程中的作用及机制  

Effect and mechanism of PRDX1 in epithelial mesenchymal transformationin of gastric cancer cells

作　　者：檀碧波[1] 李勇[1] 李淑军[2] 赵群[1] 范立侨[1] 刘庆伟[1] 赵一杰 张明月 Tan Bibo;Li Yong;Li Shujan;Zhao Qun;Fan Liqiao;Liu Qingwei;Zhao Yijie;Zhang Mingyue(The Third Department of Surgery,the Fourth Hospital of Hebei Medical University,Shijiazhuang 050019,China;Operation Room the Fourth Hospital of Hebei Medical University,Shijiazhuang 050019,China)

机构地区：[1]河北医科大学第四医院外三科,石家庄050019 [2]河北医科大学第四医院手术室,石家庄050019

出　　处：《中华肿瘤杂志》2020年第11期919-924,共6页Chinese Journal of Oncology

摘　　要：目的探讨过氧化物酶1(PRDX1)在胃癌上皮间充质转化(EMT)过程中的作用机制。方法取70例胃癌手术标本的癌及癌旁正常黏膜组织,应用免疫组化技术检测PRDX1蛋白的表达,并分析PRDX1蛋白表达与临床病理特征的关系。合成针对PRDX1基因的PRDX1-siRNA并转染人胃癌细胞株AGS,采用四甲基偶氮唑蓝(MTT)法检测细胞活性,Transwell小室实验检测细胞侵袭能力,实时荧光定量聚合酶链反应和Western blot法检测PRDX1和E-钙黏素(E-cadherin)、N-钙黏素(N-cadherin)、波形蛋白(vimentin)、紧密连接蛋白1(claudin-1)的mRNA和蛋白表达。结果胃癌组织中PRDX1蛋白表达阳性率为81.4%,高于癌旁正常黏膜(27.1%,P<0.05)。PRDX1蛋白表达与胃癌的浸润深度、淋巴结转移有关(均P<0.05)。AGS细胞中PRDX1 mRNA和蛋白的表达水平分别为2.216±0.445和1.212±0.136,均高于正常胃上皮细胞株GES-1(分别为0.342±0.041和0.328±0.038,均P<0.05)。PRDX1-siRNA转染后,AGS细胞的细胞活性明显降低(均P<0.05),LV-PRDX1-siRNA组穿膜细胞数为(112.00±17.98)个,细胞迁移率为(50.87±9.79)%,低于阴性对照组[(192.50±22.02)个、(83.03±8.67)%,均P<0.05]和空白对照组[(193.83±22.40)个、(82.40±7.21)%,均P<0.05]。LV-PRDX1-siRNA组AGS细胞中N-cadherin、vimentin、claudin-1的mRNA和蛋白表达下降,而E-cadherin表达增高(均P<0.05)。结论 PRDX1基因可能通过调控胃癌细胞的EMT促进了胃癌的进展。Objective To explore the effect and mechanism of peroxiredoxinl(PKDXl)in epithelial mesenchymal transformation(EM T)of gastric cancer cells.Methods The expression of PRDX1 protein was detected by immunohistoohemistry(1HC)in 70 paraffin specimens of cancer and normal mucosa adjacent to gastric cancer,and the relationship between PRDX1 protein and clinicopathological characteristics was analyzecl.Then PKDXl-small interfering RNA(siRNA)was synthetized and transfected into human gastric cancer cell line AGS,and 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H tetrazolium bromide(MTT)assay was used to test cell proliferation.Transwell chamber assay was employed to test invasion of cells.Real-time quantitative polymerase chain reaction(RT-qPCR)and western blot were utilized to test the expressions of PRDX1,E-cadherin,N-cadherin,vimentin,and claudin-l.Results The positive rate of PRDX1 protein expression in gastric cancer was 81.4%,higher than that in normal mucosa(27.1%,P<0.05).The expression of PRDX1 protein was related to invasive depth and lymph node metastasis of gastric cant er(P<0.05).The expressions of PRDX1 mRNA and protein in AGS cells(2.216±0.445,1.212±0.136),were higher than those in GES-1 cells(0.342±0.041,0.328±0.038)(P<0.05).When PRDX1-siRNA was transfected into AGS cells,the proliferation of AGS cells was significantly inhibited(all P<0.05).The invasion and migration rate of AGS cells in the transfection group[(112.00±17.98),(50.87±9.79)%]were significantly lower than those of the negative control group[(192.50±22.02),(83.03±8.67)%]and blank control group[(193.83±22.40),(82.40±7.21)%](all P<0.05).The expressions of mRNA and protein of N-cadherin,vimentin and claudin-l decreased,while the expression of E-cadherin increased when FRDXl-siRNA was transfected into AGS cells(P<0.05).Conclusion PRDX1 may promote the development of gastric cancer by regulating the EMT of gastric cancer cells.

关 键 词：胃肿瘤 过氧化物酶1 上皮间充质转化 临床病理特征 

分 类 号：R735.2[医药卫生—肿瘤]