Co-regulation of indole glucosinolates and camalexin biosynthesis by CPK5/CPK6 and MPK3/MPK6 signaling pathways^°°  

作　　者：Liuyi Vang Yan Zhang Rongxia Guan Sen Li Xuwen Xu Shuqun Zhang Juan Xu 

机构地区：[1]State Key Lab oratory of Plant Physiology and Biochem istry,College of Life Sciences,Zhejiang University,Hangzhou,Zhejiang 310058,China [2]Institute of Crop Sciences,Chinese Academy of Agricultural Sciences,Beijing 100081,China [3]Division of Biochemistry,University of Missouri,Columbia,MO 65211,USA

出　　处：《Journal of Integrative Plant Biology》2020年第11期1780-1796,共17页植物学报（英文版）

基　　金：This research was supported by the grants from National Natural Science Foundation of China(31922005);Zhejiang Provincial Natural Science Foundation of China(LR18C020001);the Young Elite Scientist Sponsorship Program by CAST(2018QNRC001),and 111 Project(B14027)to J.X.

摘　　要：Secondary plant metabolites,represented by indole glucosinolates(IGS)and camalexin,play important roles in Arabidopsis immunity.Previously,we demonstrated the importance of MPK3 and MPK6,two closely related MAPKs,in regulating Botrytis cinerea(Bc)-induced IGS and camalexin biosynthesis.Here we report that CPK5 and CPK6,two redundant calcium-dependent protein kinases(CPKs),are also involved in regulating the biosynthesis of these secondary metabolites.The loss-of-function of both CPK5 and CPK6 compromises plant resistance to Be.Expression profiling of CPK5-VK transgenic plants,in which a truncated constitutively active CPK5 is driven by a steroidinducible promoter,revealed that biosynthetic genes of both IGS and camalexin pathways are coordinately upregulated after the induction of CPK5-VK,leading to high-level accumulation of camalexin and 4-methoxyindole-3-ylmethylglucosinolate(4MI3G).Induction of camalexin and 4MI3G,as well as the genes in their biosynthesis pathways,is greatly compromised in cpks cpk6 mutant in response to Be.In a conditional cpk5 cpk6 mpk3 mpk6 quadruple mutant,Be resistance and induction of IGS and camalexin are further reduced in comparison to either cpks cpk6 or conditional mpk3 mpk6 double mutant,suggesting that both CPK5/CPK6 and MPK3/MPK6 signaling pathways contribute to promote the biosynthesis of 4MI3G and camalexin in defense against Be.

关 键 词：INDOLE COORDINATE CONSTITUTIVE 

分 类 号：Q943.2[生物学—植物学]