血清AFP、B7-H4联合CP检测对原发性肝癌的诊断价值  

The Value of Serum AFP,B7-H4 Combined with CP Detection in the Diagnosis of Primary Hepatic Carcinoma

作　　者：杨改标 李倩[1] 李璇[1] 龚念婷 张胜梅[1] Yang Gaibiao;Li Qian;Li Xuan;Gong Nianting;Zhang Shengmei(Department of Laboratory Pathology,32268 Troop of PLA,Dali,Yunnan 671003,China)

机构地区：[1]中国人民解放军32268部队检验病理科,云南大理671003

出　　处：《大理大学学报》2020年第10期75-79,共5页Journal of Dali University

摘　　要：目的:探究血清甲胎蛋白(AFP)、协同刺激分子B7-H4联合铜蓝蛋白(CP)检测对原发性肝癌(PHC)的诊断价值。方法:选取2017年9月至2018年9月在中国人民解放军32268部队接受治疗的42例PHC患者作为研究对象,设为PHC组,选取同期肝脏良性疾病者60例作为肝脏良性疾病组,选取同期健康体检者60例作为对照组。抽取各受试者空腹静脉血,分离血清,采用电化学发光法检测血清AFP及B7-H4含量,利用免疫透射比浊法检测血清CP含量。比较各组血清AFP、B7-H4、CP含量,探究血清AFP、B7-H4、CP及三者联合检测对PHC的诊断价值。结果:PHC组血清AFP、B7-H4及CP含量均明显高于其他两组,差异有统计学意义(P<0.05)。血清AFP、B7-H4及CP单独诊断PHC时ROC曲线的AUC分别为0.712、0.816、0.754;其对应截断值分别为103.78μg/L、50.39 ng/mL、0.31 g/L。三者联合检测时AUC为0.897,明显高于三者单独检测时的AUC,差异有统计学意义(P<0.05)。血清AFP、B7-H4及CP联合检测的灵敏度、特异度及准确度分别为92.86%、93.33%和93.21%,均高于各指标单项检测时。结论:血清AFP、B7-H4及CP联合检测可明显提高对PHC的诊断率,对临床诊断PHC具有重要的意义。Objective:To explore the diagnostic value of serum alpha-fetoprotein(AFP),co-stimulatory molecule B7-H4 combined with ceruloplasmin(CP)for primary hepatic carcinoma(PHC).Methods:Forty-two PHC patients who were treated in the 32268 Troop of People's Liberation Army during the period from September 2017 to September 2018 were selected as the PHC group,60 patients with benign liver disease were selected as the benign liver disease group,and another 60 healthy controls during the same period were selected as the control group.The fasting venous blood in each subject was collected to separate serum.The contents of serum AFP and B7-H4 were detected by electrochemiluminescence method.The content of serum CP was detected by immunity transmission turbidity.The levels of serum AFP,B7-H4 and CP were compared among all groups.The diagnostic value of serum AFP,B7-H4,CP and their combination for PHC was explored.Results:The contents of serum AFP,B7-H4 and CP in the PHC group were significantly higher than those in the benign liver disease group and the control group(P<0.05).The area under the ROC curve(AUC)values of serum AFP,B7-H4 and CP for diagnosis of PHC were 0.712,0.816 and 0.754,and their corresponding cut-off values were 103.78μg/L,50.39 ng/mL and 0.31 g/L,respectively.AUC of their combination detection was 0.897,which was significantly higher than that of single index(P<0.05).The sensitivity,specificity and accuracy of serum AFP combined with B7-H4 and CP detection were 92.86%,93.33%and 93.21%,which were higher than those of single index.Conclusion:The combination detection of serum AFP,B7-H4 and CP can significantly improve diagnosis rate of PHC,which is of great significance for clinical diagnosis of PHC.

关 键 词：甲胎蛋白 协同刺激分子 铜蓝蛋白 原发性肝癌 联合诊断 

分 类 号：R735.7[医药卫生—肿瘤]