根癌农杆菌介导的巨大口蘑遗传转化体系的构建  

Construction of Agrobacterium-mediated transformation system in Macrocybe gigantea

作　　者：查丽燕 宋舒晴 王越 文华枢 莫美华[1] ZHA Li-Yan;SONG Shu-Qing;WANG Yue;WEN Hua-Shu;MO Mei-Hua(College of Food Science,South China Agricultural University,Guangzhou,Guangdong 510642,China)

机构地区：[1]华南农业大学食品学院,广东广州510642

出　　处：《菌物学报》2020年第10期1897-1904,共8页Mycosystema

基　　金：国家自然科学基金(31272218);广东省科技创新战略专项资金(重点领域研发计划项目)(2018B020206001);广东省现代农业产业技术体系创新团队建设——食用菌产业技术体系创新团队(2019KJ103)。

摘　　要：以巨大口蘑菌丝为受体材料,利用含有双元质粒plasmid4的根癌农杆菌EHA105介导,首次成功建立了巨大口蘑的遗传转化体系。通过潮霉素抗性筛选、PCR鉴定和绿色荧光蛋白的检测,表明潮霉素抗性基因(Hyg)已经整合到巨大口蘑基因组中,增强型绿色荧光蛋白基因(eGFP)在巨大口蘑菌丝中获得表达,并能够稳定遗传。本研究建立了农杆菌介导的巨大口蘑遗传转化体系,为今后巨大口蘑的基因功能研究奠定了基础。The transformation system of Macrocybe gigantea was successfully established for the first time by using Agrobacterium tumefaciens EHA105 bearing binary plasmid plasmid4.The results of hygromycin resistance screening,PCR identification and fluorescence microscopy analysis indicated that Hyg was integrated into M.gigantea genome,and enhanced green fluorescent protein gene(eGFP)was expressed in M.gigantea.The development of Agrobacterium-mediated genetic transformation system can facilitate further gene function research in M.gigantea.

关 键 词：巨大口蘑 根癌农杆菌 遗传转化 潮霉素 增强型绿色荧光蛋白基因 

分 类 号：S646[农业科学—蔬菜学]