川桑中桑皮素的提取工艺优化  

Optimized Extraction of Mulberrin from Morus Notabilis Schneid

作　　者：左亚奇 高志红 庞策 孙转友 李芳 甄攀[1] ZUO Ya-qi;GAO Zhi-hong;PANG Ce;SUN Zhuan-you;LI Fang;ZHEN Pan(Hebei North University,Zhangjiakou,075000,China;Guangzong County Hospital of Traditional Chinese Medicine,Xingtai,054600,China;Beijing University of Chinese Medicine DongFang Colege,Langfang,065000,China)

机构地区：[1]河北北方学院,张家口075000 [2]广宗县中医医院,邢台054600 [3]北京中医药大学东方学院,廊坊065000

出　　处：《神经药理学报》2020年第1期20-23,共4页Acta Neuropharmacologica

基　　金：河北省高等学校科学技术研究重点项目(No:ZD2016016)。

摘　　要：目的:探讨川桑中桑皮素的最佳提取工艺。方法:以桑皮素含量为指标,利用4因素3水平L9(34)正交试验,分析乙醇浓度、提取时间、溶媒倍数、提取次数对桑皮素提取效率的影响。HPLC法测定桑皮素含量:YMCPack ODS-A色谱柱(5μm,4.6 mm×250 mm);流动相为甲醇-水(80:20);柱温25℃;流速1.0 mL·min-1;检测波长268 nm。结果:最佳提取工艺:提取前提取溶剂浸泡3 h,回流提取3次,每次提取时间均为0.5 h,提取溶媒倍数均为20倍。最优提取条件下测得川桑中桑皮素含量为1.510 mg·g-1。结论:乙醇浓度、溶媒倍数、提取次数对桑皮素提取效率均有显著影响,提取时间无显著影响。Objective:To investigate the optimum extraction technology of mulberrin from Morus notabilis Schneid.Methods:Taking the content of mulberrin as an index,the effects of ethanol concentration,extraction time,solvent multiple,extraction times on the extraction efficiency of mulberrin was investigated by 4-Factor-3-Level orthogonal experiment.HPLC was used to detect the extraction efficiency of mulberrin on YMC-Pack ODS-A column(4.6 mm×250 mm,5μm)with water-methanol(20:80)as mobile phase.The flow rate was 1.0 mL·min-1,the column temperature was 25℃and the wavelength of UV detector was 268 nm.Results:The optimum extraction technology:before extraction,Morus notabilis was soaked in extraction solvent for 3.0 h,and extracted with reflux for 3 times,each extraction time was 0.5 h,and the extraction solvent multiple was 20 times.Under optimal extraction conditions,the content of mulberry in Morus notabilis Schneid was 1.510 mg·g-1.Conclusion:Ethanol concentration,solvent multiple and extraction times all had significant effects on the extraction efficiency of mulberry,and the extraction time didn’t have significant influences on the extraction efficiency of mulberry.

关 键 词：正交试验 川桑 桑皮素 提取工艺 HPLC 

分 类 号：R284[医药卫生—中药学]