KIFC3在结肠癌中的表达及其对结肠癌细胞迁移和侵袭的影响  

Expression of KIFC3 in colon cancer and its effect on migration and invasion of colon cancer cells

作　　者：张岚[1] 董卫国[1] ZHANG Lan;DONG Weiguo(Department of Gastroenterology,Renmin Hospital of Wuhan University,Wuhan 430060,China)

机构地区：[1]武汉大学人民医院消化内科,湖北武汉430060

出　　处：《胃肠病学和肝病学杂志》2020年第11期1220-1225,共6页Chinese Journal of Gastroenterology and Hepatology

摘　　要：目的探讨驱动蛋白家族分子C3(KIFC3)在结肠癌组织中的表达及其对结肠癌细胞迁移和侵袭的影响。方法GEPIA数据库分析KIFC1、KIFC2和KIFC3在食管癌、胃癌和结肠癌组织及相应正常组织的差异表达和对患者生存时间的影响;STRING数据库与Cytoscape软件联合分析KIFC3可能参与的生物学过程;TIMER数据库分析KIFC3与常见细胞增殖、周期、迁移和侵袭相关分子标志物表达量的关系;细胞划痕与Transwell小室实验检测KIFC3对结肠癌细胞迁移与侵袭的影响;Western blotting实验检测KIFC3在NCM460与结肠癌细胞中的表达差异并探索KIFC3影响结肠癌细胞迁移和侵袭的潜在分子机制。结果KIFC3在结肠癌组织中显著高表达,与结肠癌患者不良预后紧密相关。基因富集分析表明,KIFC3参与细胞间黏附和紧密连接等生物学过程,且与基质金属蛋白酶-2(matrix metalloproteinase-2,MMP2)、MMP9相关性显著。干扰KIFC3表达能够抑制结肠癌细胞迁移与侵袭,同时可以抑制上皮间质转化(epithelial mesenehymal transition,EMT)的发生。结论KIFC3可能通过EMT参与结肠癌细胞转移,影响患者生存,有望成为治疗结肠癌的潜在靶点。Objective To investigate the expression of KIFC3 in colon cancer tissue and its potential effect on migration and invasion of colon cancer cells.Methods GEPIA database was used to analyze the differential expressions of KIFC1,KIFC2 and KIFC3 between esophageal cancer,gastric cancer and colon cancer tissues and the corresponding normal tissues,and their effects on the survival time of patients.STRING database and Cytoscape software were used to analyze the biological processes that KIFC3 might be involved in.TIMER database was used to analyze the relationship between KIFC3 and the expressions of molecular markers related to cell proliferation,cell cycle,migration and invasion.Effect of KIFC3 on migration and invasion of colon cancer cells detected by cell scratch test and Transwell chamber test.Western blotting was used to detect the expression of KIFC3 in NCM460 cells and colon cancer cells,and to explore the potential molecular mechanism of KIFC3 affecting the migration and invasion of colon cancer cells.Results KIFC3 was highly expressed in colon cancer tissue,which was closely related to the poor prognosis of patients with colon cancer.Gene enrichment analysis showed that KIFC3 was involved in the biological processes of intercellular adhesion and tight junction,and was significantly correlated with matrix metalloproteinase-2(MMP2)and MMP9.Interference of KIFC3 expression could inhibit the migration and invasion of colon cancer cells,and inhibit the occurrence of epithelial mesenchymal transition(EMT).Conclusion KIFC3 may participate in the metastasis of colon cancer cells through EMT and affect the survival of patients,which is expected to become a potential target for the treatment of colon cancer.

关 键 词：KIFC3 结肠癌 EMT 迁移 侵袭 

分 类 号：R735.3[医药卫生—肿瘤]