Linifanib对胃癌模型大鼠胃黏膜组织细胞增殖、凋亡及周期分布的影响  

Effects of Linifanib on the proliferation,apoptosis and cycle distribution of gastric mucosal tissue cells in gastric cancer model rats

作　　者：黎玉容 彭颂兴 杨兵 LI Yurong;PENG Songxing;YANG Bing(Department of Gastroenterology,Shenzhen Ninth People’s Hospital,Shenzhen 518116,China)

机构地区：[1]深圳市第九人民医院消化内科,广东深圳518116

出　　处：《胃肠病学和肝病学杂志》2020年第11期1261-1265,共5页Chinese Journal of Gastroenterology and Hepatology

基　　金：深圳市卫生局科研项目资助(201845331)。

摘　　要：目的探究利尼伐尼(Linifanib)对胃癌模型大鼠胃黏膜组织细胞增殖、凋亡及周期分布的影响。方法选取35只SD健康雄性大鼠,其中5只为正常组,其余30只建模,并分为模型组、药物对照组、Linifanib组,对各组大鼠分别进行干预。酶联免疫吸附实验法(ELISA)检测胃动素(MTL)、胃泌素(GAS)水平,观察各组大鼠胃黏膜组织细胞增殖、凋亡及周期分布,检测周期分布、细胞凋亡蛋白表达。结果Linifanib组大鼠MTL、GAS水平均高于模型组、药物对照组(P<0.05)。Linifanib组大鼠胃黏膜组织细胞增殖率低于模型组、药物对照组,凋亡率高于模型组、药物对照组(P<0.05)。Linifanib组G1期细胞比例低于正常组,高于模型组、药物对照组(P<0.05)。Linifanib组大鼠p-Akt、mTOR、Bcl-2、caspase3相对表达量均高于正常组,低于模型组、药物对照组,PTEN、Bax相对表达量低于正常组,高于模型组、药物对照组(P<0.05)。结论使用Linifanib对胃癌大鼠进行干预,能够改善胃癌大鼠胃功能,调控p-Akt、mTOR、PTEN及Bcl-2、Bax、caspase3蛋白表达,从而对大鼠胃黏膜组织增殖、凋亡及周期分布进行调控。Objective To investigate the effects of Linifanib on the proliferation,apoptosis and cycle distribution of gastric mucosal tissue cells in gastric cancer model rats.Methods Thirty-five SD healthy male rats were selected,of which 5 were normal group and the remaining 30 were modeled and divided into model group,drug control group and Linifanib group,and the rats in each group were intervened separately.The levels of motilin(MTL)and gastrin(GAS)were detected by enzyme-linked immunosorbent assay(ELISA),and the proliferation,apoptosis and cell cycle distribution of gastric mucosa tissues were observed in each group of rats,and the cycle distribution and apoptotic protein expression were detected.Results The levels of MTL and GAS in Linifanib group were higher than those in model group and drug control group(P<0.05).The proliferation rate of gastric mucosal tissue cells in Linifanib group was lower than that in model group and drug control group,and the apoptosis rate was higher than that in model group and drug control group(P<0.05).The percentage of cells in G1 phase in Linifanib group was lower than that in normal group,higher than that in model group and drug control group(P<0.05).The relative expression of p-Akt,mTOR,Bcl-2 and caspase3 in Linifanib group were higher than that in normal group,lower than that in model group and drug control group,and the relative expressions of PTEN and Bax were lower than that in normal group,higher than that in model group and drug control group(P<0.05).Conclusion Intervention with Linifanib in gastric cancer rats can improve gastric function of gastric cancer rats,regulate the expressions of p-Akt,mTOR,PTEN and Bcl-2,Bax,caspase3 proteins,and thus regulate the proliferation,apoptosis and cycle distribution of gastric mucosal tissue in rats.

关 键 词：胃癌 利尼伐尼 凋亡 细胞周期 胃功能 

分 类 号：R735.2[医药卫生—肿瘤]