山茱萸中具有抗氧化活性的环烯醚萜类成分  

Iridoids with Antioxidant Activity from Cornus officinalis

作　　者：栾娜 钟祥键 王欣 冀麟麟 李金杰[1] 尚小雅[1] Luan Na;Zhong Xiangjian;Wang Xin;Ji Linlin;Li Jinjie;Shang Xiaoya(Beijing Key Laboratory of Bioactive Substance and Functional Foods,Beijing Union University,Beijing 10019)

机构地区：[1]北京联合大学生物活性物质与功能北京市重点实验室,北京100191

出　　处：《中国食品学报》2020年第11期255-264,共10页Journal of Chinese Institute Of Food Science and Technology

基　　金：北京市教委科技计划一般项目(No.KZ201911417014)。

摘　　要：采用正相硅胶柱色谱、Sephadex LH-20凝胶柱色谱、大孔吸附树脂柱色谱、氰基柱色谱和高效液相色谱等方法对山茱萸乙醇提取物中具有抗氧化活性的环烯醚萜类组分进行分离纯化;采用核磁共振波谱技术和质谱技术鉴定其结构;通过总抗氧化能力FRAP法测定其抗氧化活性。结果表明:共分离并鉴定出4个具有抗氧化活性的环烯醚萜类成分,分别为:马钱子苷(1)、马钱素-7-酮(2)、1-β-甲氧基马钱子苷元(3)和1-去羟基马钱子苷元(4),其中化合物3和4是首次从山茱萸属中分离得到的新的天然产物。To isolate and purify the iridoids with antioxidant activity from ethanol extract of the Cornus officinalis,macroporous resin,normal phase silica gel,low pressure normal phase silican gel and cyanopropyl silica,Sephadex LH-20 and preparative HPLC column chromatography were used.The structures of the compounds were identified on the basis of spectral methods including NMR,MS and comparison with literature.The all compounds were evaluated the antioxidant activity using FRAP method and four iridoids with antioxidant activity were identified as loganin(1),ketologanin(2),1-β-O-methyl loganin aglucone(3)and 1-deoxyloganin aglycone(4).Compounds 3 and 4 were new natural products and reported in this species for the first time.

关 键 词：山茱萸 环烯醚萜 分离 鉴定 抗氧化 

分 类 号：R284[医药卫生—中药学]