UCF-101对蛛网膜下腔出血大鼠神经功能的保护作用  

The protective effect of UCF-101 on neurological function of rats with subarachnoid hemorrhage

作　　者：程振宇[1] 申屠华松 陈亦华[1] 何忠平 俞北伟 王瑞权[4] 傅斌[1] CHENG Zhenyu;SHENTU Huasong;CHEN Yihua;HE Zhongping;YU Beiwei;WANG Ruiquan;FU Bin(Department of Neurosurgery,Jinhua People’s Hospital,Jinhua 321000,China;Jinhua Institute of Food&Drug Inspection and Testing,Jinhua 321000,China;Department of Clinical Medical Laboratory,Jinhua People’s Hospital,Jinhua 321000,China;Department of Pathology,Jinhua People’s Hospital,Jinhua 321000,China)

机构地区：[1]金华市人民医院神经外科,浙江金华321000 [2]金华市食品药品检验检测研究所,浙江金华321000 [3]金华市人民医院临床医学检验科,浙江金华321000 [4]金华市人民医院病理科,浙江金华321000

出　　处：《温州医科大学学报》2020年第11期906-910,914,共6页Journal of Wenzhou Medical University

基　　金：金华市科技计划项目(2019-4-031)。

摘　　要：目的:探讨UCF-101对蛛网膜下腔出血(SAH)大鼠神经功能的保护作用。方法:90只大鼠随机分成Sham组、SAH组和UCF-101组,每组30只。采用枕大池二次注血法制作大鼠SAH动物模型,按照3.0μmol/kg剂量给予UCF-101组大鼠腹腔注射UCF-101。采用Western blot法检测各组10只大鼠脑组织pro-caspase-3、pro-caspase-9、剪切PARP、caspase-3和caspase-9蛋白表达,采用TUNEL法检测各组10只大鼠凋亡神经细胞,记录各组10只大鼠Morris水迷宫定位航行实验逃避潜伏期和Kaoutzanis M神经行为学评分。结果:SAH组大鼠脑组织pro-caspase-3、pro-caspase-9、剪切PARP、caspase-3和caspase-9蛋白表达量、凋亡神经细胞比例和逃避潜伏期较Sham组大鼠显著升高(均P<0.05),而SAH组大鼠Kaoutzanis M神经行为学评分较Sham组大鼠显著降低(P<0.05);与SAH组大鼠比较,UCF-101组大鼠上述各指标均发生不同程度的逆转(均P<0.05)。结论:UCF-101可以显著抑制SAH大鼠神经细胞凋亡,改善SAH大鼠认知功能和神经行为能力,对SAH大鼠起到明显的神经功能保护作用。Objective:To examine the protective effect of UCF-101 on neurological function of rats with subarachnoid hemorrhage(SAH).Methods:A total of 90 rats were randomly assigned to Sham group,SAH group and UCF-101 group,with 30 rats in each group containing.The rat SAH animal model was established via double autologous blood injection into cisterna magna.Rats in the UCF-101 group were administrated intraperitoneally with UCF-101(3.0μmol/kg body weight).The western-blotting assay was used to determine the expressions of pro-caspase-3,pro-caspase-9,cleaved PARP,caspase-3 and caspase-9 in brain tissues of 10 rats in each group.The TUNEL staining was performed to investigate apoptotic neuronal cells in 10 rats of each group.Escape latency via the place navigation of Morris water maze and Kaoutzanis M’s neurobehavioral score were recorded of 10 rats from each group.Results:The brain tissue expressions of pro-caspase-3,pro-caspase-9,cleaved PARP,caspase-3 and caspase-9,percentage of apoptotic neuronal cells as well as escape latency of rats in SAH group were significantly higher than those of rats in Sham group(all P<0.05);while Kaoutzanis M’s neurobehavioral score were substantially lower in SAH group than in Sham group(P<0.05).As compare with SAH group,all the preceding variables were remarkably reversed to different extents in UCF-101 group(all P<0.05).Conclusion:UCF-101 can markedly inhibit the neuronal cellular apoptosis of SAH rats,and improve their cognitive function and neurobehavioral ability,and thereby exert a protective effect on neurological function of SAH rats.

关 键 词：UCF-101 蛛网膜下腔出血 神经保护 大鼠 

分 类 号：R737.15[医药卫生—肿瘤]