Applying Both Chemical Liquefaction and Enzymatic Catalysis Can Increase Production of Agaro-Oligosaccharides from Agarose  

作　　者：JIANG Chengcheng LIU Zhen LIU Jie SUN Jianan XU Jiachao LI Laihao MAO Xiangzhao 

机构地区：[1]College of Food Science and Engineering,Ocean University of China,Qingdao 266003,China [2]Laboratory for Marine Drugs and Bioproducts of Qingdao National Laboratory for Marine Science and Technology,Qingdao 266237,China [3]Key Laboratory of Aquatic Product Processing,Ministry of Agriculture,South China Sea Fisheries Research Institute,Chinese Academy of Fishery Science,Guangzhou 510300,China

出　　处：《Journal of Ocean University of China》2020年第6期1371-1377,共7页中国海洋大学学报（英文版）

基　　金：This work was supported by the National Key R&D Program of China(No.2018YFC0311200);the Fundamental Research Funds for the Central Universities(No.201941002);the Taishan Scholars Project(No.tsqn201812020).

摘　　要：Red algae represents an important marine bioresource.One high-value utilization of red algae is the production of agarooligosaccharides which have many positive biological effects.However,the lack of an efficient production route seriously limits the application of agaro-oligosaccharides.In this study,we established a green route that combines chemical liquefaction and enzymatic catalysis for the efficient production of agaro-oligosaccharides,and we used the production of neoagarotetraose(NA4)as an example.Agarose(150 g L^−1)liquefaction by citric acid was controlled with respect to two targets:a 100%liquefaction rate and a high average degree of polymerization(>4)of the liquesced agaro-oligosaccharides,which were then catalyzed byβ-agarase into an oligosaccharides mixture with a high concentration of NA4(30.8 g L^−1)in a 1-L reaction volume.After purification,we obtained 25.5 g of NA4 with a purity of 92%.This work establishes an easy route for the efficient production of pure agaro-oligosaccharides from agarose.

关 键 词：neoagarotetraose AGAROSE AGARASE marine polysaccharide expression 

分 类 号：O636.1[理学—高分子化学]