云南高校图书馆联盟文献共享服务平台- AGARASE

AGARASE: 作品数：14被引量：49H指数：4; 导出分析报告; 相关领域：生物学更多>>; 相关作者：牟宗娟李贵阳莫照兰茅云翔孔凡娜更多>>; 相关机构：中国海洋大学黄海水产研究所中国科学院更多>>; 相关期刊：《Journal of Ocean University of China》《High Technology Letters》《海洋科学》《Journal of Marine Science》更多>>; 相关基金：国家自然科学基金国家高技术研究发展计划福建省自然科学基金公益性行业(农业)科研专项更多>>

Structural basis for the cold activation and adaptation of anα-agarase from marine bacterium Catenovulum agarivorans STB13被引量：1: 《Food Bioscience》2023年第3期1471-1478,共8页Wenyan Xie Yuxian You Xiaofeng Ban Anqi Zhang Caiming Li Zhengbiao Gu Zhaofeng Li; financially supported by the National Key R&D Pro-gram of China(2019YFD0901901);the National Natural Science Foundation of China(No.32272263);Science and Technology Support Program(Modern Agriculture)of Jiangsu Province(BE2022323).; Enzymes have high activity at low temperatures are defined as cold-adapted enzymes,but few enzymes show an activation effect after low-temperature incubation.In this study,we cloned an agarase from the marine bacte-ri...; 关键词：α-agarase Cold activation Cold adaptation Carbohydrate-binding module LINKER Agaro-oligosaccharides

Agarase Production by Marine Pseudoalteromonas sp.MHS:Optimization,and Purification: 《Journal of Marine Science》2022年第1期11-22,共12页Mona M.Sharabash Samia S.Abouelkheir Mona E.M.Mabrouk Hanan A.Ghozlan Soraya A.Sabry; Agar is an essential polysaccharide that has been utilized in numerous fields.Many kinds of literature have been published regarding agarolytic microorganisms’isolation and agarases biochemical studies.In this search...; 关键词：AGARASE Ulva lactuca OPTIMIZATION Pseudoalteromonas sp.MHS Red seaweed utilization

Applying Both Chemical Liquefaction and Enzymatic Catalysis Can Increase Production of Agaro-Oligosaccharides from Agarose: 《Journal of Ocean University of China》2020年第6期1371-1377,共7页JIANG Chengcheng LIU Zhen LIU Jie SUN Jianan XU Jiachao LI Laihao MAO Xiangzhao; This work was supported by the National Key R&D Program of China(No.2018YFC0311200);the Fundamental Research Funds for the Central Universities(No.201941002);the Taishan Scholars Project(No.tsqn201812020).; Red algae represents an important marine bioresource.One high-value utilization of red algae is the production of agarooligosaccharides which have many positive biological effects.However,the lack of an efficient prod...; 关键词：neoagarotetraose AGAROSE AGARASE marine polysaccharide expression

Overexpression and characterization of a thermostable β-agarase producing neoagarotetraose from a marine isolate Microbulbifer sp.AG1被引量：1: 《Acta Oceanologica Sinica》2019年第2期96-106,共11页Yanbing Zhu He Gao Hebin Li Hui Ni Zedong Jiang Lijun Li Anfeng Xiao; The Natural Science Foundation of Fujian Province of China under contract No.2016J01162;the Program for New Century Excellent Talents in Fujian Province University,China under contract No.B15139; An agarase gene containing 1 302 bp was cloned from Microbulbifer sp. AG1. It encoded a mature protein of 413 amino acids plus a 20-residue signal peptide. The recombinant enzyme without the signal peptide was express...; 关键词：THERMOSTABLE β-agarase neoagarotetraose Microbulbifer sp.

Enhanced a novel β-agarase production in recombinant Escherichia coli BL21(DE3) through induction mode optimization and glycerol feeding strategy: 《Acta Oceanologica Sinica》2018年第2期110-118,共9页CHAN Zhuhua CHEN Xinglin HOU Yanping GAO Boliang ZHAO Chungui YANG Suping ZENG Runying; The Public Science and Technology Research Funds Projects of Ocean under contract No.201505026;the Fujian Province Natural Science Foundation under contract Nos 2016J01160 and 2017N0015;the Scientific Research Foundation of Third Institute of Oceanography,SOA under contract No.2016038; Agarases are hydrolytic enzymes that act on the hydrolysis of agar and have a broad range of applications in food,cosmetics and pharmaceutical industries. In this study, a glycerol feeding strategy based on induction ...; 关键词：β-agarase Escherichia coli process optimization glycerol feeding strategy neoagarooligosaccharide

Deletion of a Non-Catalytic Region Increases the Enzymatic Activity of a β-Agarase from Flammeovirga sp. MY04被引量：2: 《Journal of Ocean University of China》2015年第5期841-848,共8页HAN Wenjun GU Jingyan LIU Huihui LI Fuchuan WU Zhihong LI Yuezhong; financially supported by the Open Research Fund Program of Shandong Provincial Key Laboratory of Glycoscience&Glycotechnology(Ocean University of China)KLGG(OUC)201301;the National Natural Science Foundation of China Grants 31300664 and 31130004;the State Key Laboratory of Microbial Technology Grant(Shandong University)M2013-11; A Glycoside hydrolase （GH） typically contains one catalytic module and varied non-catalytic regions （NCRs）. However, effects of the NCRs to the catalytic modules remain mostly unclear except the carbohydrate-bindi...; 关键词：AGARASE enzymatic characteristic Flammeovirga non-catalytic region OLIGOSACCHARIDE TRUNCATION

Expression and enzymatic characterization of a cold-adapted β-agarase from Antarctic bacterium Pseudoalteromonas sp.NJ21被引量：4: 《Chinese Journal of Oceanology and Limnology》2015年第2期319-327,共9页李江沙玉杰; Supported by the Public Science and Technology Research Funds Project of Ocean(No.201105027);the Shandong Province Young and the Middle-Aged Scientists Research Awards Fund(No.DS2010HZ001);the Basic Scientific Research Funds of First Institute of Oceanography,State Oceanic Administration(No.GY02-2011G17); An agar-degrading bacterium, designated as Pseudoalteromonas sp. NJ21, was isolated from an Antarctic sediment sample. The agarase gene aga1161 from Pseudoalteromonas sp. NJ21 consisting of a 2 382-bp coding region wa...; 关键词：Antarctic bacterium Pseudoalteromonas AGARASE EXPRESSION enzymatic characterization

4株琼胶降解菌的分离、鉴定及产酶条件分析被引量：7: 《海洋科学》2013年第4期13-20,共8页牟宗娟李贵阳茅云翔孔凡娜莫照兰; 国家自然科学基金资助项目(30972247,30700621);国家“863”高技术研究发展计划(2012AA10A411,2012AA10A406,2012AA100815);农业部公益行业专项(200903030);国家科技基础条件平台-水产种质资源平台; 从海藻及海参中分离筛选得到4株琼胶降解菌HD、JL、QJ和HS,均为革兰氏阴性菌,确定了它们发酵产酶的最佳条件均为:2216E海水培养基、初始Ph 7.5、琼胶底物浓度0.30%、培养温度28℃、培养时间36 h。大部分酶分泌到细菌细胞外。生理生化检...; 关键词：琼胶降解菌(agarase-producing bacteria) 琼胶酶活力鉴定白色噬琼胶菌(Agarivor ansalbus) β-琼胶酶基因

A Polysaccharide-Degrading Marine Bacterium Flammeovirga sp.MY04 and Its Extracellular Agarase System被引量：5: 《Journal of Ocean University of China》2012年第3期375-382,共8页HAN Wenjun GU Jingyan YAN Qiujie LI Jungang WU Zhihong GU Qianqun LI Yuezhong; supported by the Scientific Research Fund of the Sichuan Provincial Education Department(09ZA181);by grants from the State Key Laboratory of Microbial Technology (M2010-12);the National Science Foundation of China (30870001); Bacteria of the genus Flammeovirga can digest complex polysaccharides (CPs), but no details have been reported regarding the CP depolymerases of these bacteria. MY04, an agarolytic marine bacterium isolated from coast...; 关键词：Flammeovirga polysaccharide degradation extracellular agarase system neoagaro-oligosaccharide

Identification of a marine agarolytic bacterium Agarivorans albus QM38 and cloning and sequencing its beta-agarase genes被引量：5: 《Acta Oceanologica Sinica》2011年第1期118-124,共7页DU Zongjun WANG Jing YANG Lijun CHEN Guanjun; Shandong Provincial Natural Science Foundation,China under contract No. ZR2009EQ009;Independent Innovation Foundation of Shandong University (IIFSDU);Key Lab of Marine Bioactive Substance and Modern Analytical Technique,SOA,China under contract No. MBSMAT-2009-07; A total of 117 agar-decomposing cultures were isolated from coastal seawater around Qingdao, China. The phenotypic and agarolytic features of an agarolytic isolate, QM38, were investigated. The strain was gram negativ...; 关键词：marine bacteria isolation and characterization AGARASE gene cloning Agarivoransalbus

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