牛磺酸对甲醛致性成熟雄性大鼠生殖毒性具有保护作用  

Protective effect of taurine against formaldehyde-induced male reproductive toxicity in adult male rats

作　　者：耿天 孙发 田源 肖跃海 孙超 刘焙 GENG Tian;SUN Fa;TIAN Yuan;XIAO Yue-hai;SUN Chao;LIU Bei(Guizhou Medical University,Guiyang,Guizhou 550004,China;Guizhou Provincial People Hospital,Guiyang,Guizhou 550002,China;Department of Urology,The Affiliated Hospital of Guizhou Medical University,Guiyang,Guizhou 550004,China)

机构地区：[1]贵州医科大学,贵州贵阳550004 [2]贵州省人民医院,贵州贵阳550002 [3]贵州医科大学附属医院泌尿外科,贵州贵阳550004

出　　处：《中华男科学杂志》2020年第9期777-782,共6页National Journal of Andrology

摘　　要：目的:研究牛磺酸对甲醛致性成熟大鼠生殖毒性损伤的作用及影响。方法:选用SD雄性性成熟大鼠48只,随机平均分为对照组、甲醛染毒组、牛磺酸干预组、牛磺酸干预+甲醛染毒组,每组大鼠12只。甲醛染毒采用腹腔注射10 mg/(kg·2 d),牛磺酸干预采用灌胃方式100 mg/(kg·d),对照组每天给予正常喂养+生理盐水灌胃,持续30 d。结束后抽取大鼠腹主静脉血液并摘取睾丸,称量所有大鼠体重和睾丸重量,进行睾丸脏器系数的比较;选取一侧睾丸组织进行病理学HE染色;所抽取的静脉血,离心后取血清,检测各组血清激素;用western印迹方法检测Bcl-2、Bax蛋白的表达情况。结果:各组大鼠体重、睾丸重量及睾丸脏器系数无明显差异(P>0.05)。甲醛组大鼠的睾丸生精细胞减少,生精小管层次紊乱,结构松散。牛磺酸干预后基本恢复正常。牛磺酸单独干预组与正常对照组相比未见明显异常。甲醛组血清激素水平中睾酮(T)、黄体生成素(LH)和卵泡刺激素(FSH)明显降低(P<0.05),促性腺激素释放激素(GnRH)水平显著升高(P<0.01),差异有统计学意义。牛磺酸干预后血清激素水平较甲醛组有所恢复,差异有统计学意义(P<0.05)。牛磺酸干预后,甲醛组凋亡蛋白Bax水平明显升高,基本达到正常水平,差异有统计学差异(P<0.05)。结论:牛磺酸对于甲醛导致的性成熟大鼠生殖毒性有一定的保护效果。Objective:To study the effect of taurine on the reproductive toxicity damage induced by formaldehyde(FM)in adult male rats.Methods:Forty-eight SD adult male rats were equally randomized into a normal control,an FM poisoning(FMP),a taurine intervention(TI),and an TI+FMP group.The control rats were given normal diet and gavage of saline,the rats of the FMP group treated intraperitoneally with FM at 10 mg/kg qd alt,those of the TI group intragastrically with taurine at 100 mg/kg qd,and those of the TI+FMP group with both FM and taurine at the above doses.After 30 days of treatment,the blood of the abdominal cardinal vein of the rats was extracted for measurement of the levels of serum hormones,the body weight,testis weight and testicular coefficient obtained,the testis tissue subjected to HE staining,and the expressions of Bcl-2 and Bax determined by Western blot.Results:There were no statistically significant differences among the four groups of rats in the body weight,testis weight or testicular coefficient(P>0.05).The rats in the FMP group showed obviously decreased testicular spermatogenic cells and disordered layers and loose structure of seminiferous tubules,which were basically restored to normal after taurine intervention.Compared with the normal controls,the animals of the TI group exhibited no significant abnormality,but those of the FMP group presented markedly reduced levels of serum T,LH and FSH(P<0.05),and dramatically increased level of Gonadotropin-releasing hormone(GnRH)(P<0.01).The levels of serum hormones were all significantly improved(P<0.05)and that of the apoptotic protein Bax basically returned to normal(P<0.05)after taurine intervention.Conclusion:Taurine has a certain protective effect against male reproductive toxicity caused by formaldehyde.

关 键 词：甲醛 牛磺酸 雄性生殖毒性 细胞凋亡 

分 类 号：R698.2[医药卫生—泌尿科学]