Nd:YAG激光对钛种植体表面影响的研究  

Study on the effect of Nd:YAG water-cooled laser on the surface of titanium implant

作　　者：葛琳华[1] 孙梦君[1] 束蓉[1] GE Lin-hua;SUN Meng-jun;SHU Rong(Department of Periodontology,Shanghai Ninth People's Hospital,College of Stomatology,Shanghai Jiao Tong University School of Medicine,National Clinical Research Center for Oral Diseases,Shanghai Key Laboratory of Stomatology&Shanghai Research Institute of Stomatology,Shanghai 200011,China)

机构地区：[1]上海交通大学医学院附属第九人民医院·口腔医学院牙周病科,国家口腔疾病临床医学研究中心,上海市口腔医学重点实验室/上海市口腔医学研究所,上海200011

出　　处：《上海交通大学学报（医学版）》2020年第10期1371-1375,共5页Journal of Shanghai Jiao tong University:Medical Science

基　　金：上海交通大学医学院高水平地方高校创新团队(SSMU-ZDCX20180900)。

摘　　要：目的·通过研究Nd:YAG水雾激光对钛种植体表面的影响,评估其应用在种植体周围炎治疗中的安全性。方法·使用2种不同的钛片(机械抛光MP和喷砂酸蚀SLA),随机分为Nd:YAG激光处理组(功率1.5 W,水2气3,光纤直径400μm,非接触模式)、Er,Cr:YSGG激光处理组(功率1.5 W,频率20 Hz,模式S,水10%,气15%,光纤头MZ6,非接触模式)及空白对照组。激光处理后,通过扫描电子显微镜(scanning electron microscope,SEM)观察激光辐照在这些表面上的影响。并通过免疫荧光观察细胞黏附数量,real-time PCR检测细胞黏附基因表达。结果·激光处理组SEM结果未发现钛片表面形态的改变;各组钛片表面细胞黏附数量及基因表达差异无统计学意义(P>0.05),激光处理对其没有影响。结论·Nd:YAG水雾激光消毒种植体对钛种植体表面没有影响,在种植体周围炎治疗中是安全的。Objective·To study the effect of Nd:YAG water-cooled laser on the titanium implant surface,and evaluate its safety in the treatment of peri-implantitis.Methods·Two different titanium sheets(mechanically polished and sandblasted and acid etched)were used and randomly divided into three groups:Nd:YAG laser treatment group(power 1.5 W,water 2 gas 3,fiber diameter 400μm,non-contact mode),Er,Cr:YSGG laser treatment group(power 1.5 W,frequency 20 Hz,mode S,water 10%,gas 15%,optical fiber MZ6,non-contact mode)and blank control group.After laser treatment,the effect of laser irradiation on these surfaces was observed by scanning electron microscope(SEM).The amount of cell adhesion was observed by immunofluorescence,and real-time PCR was used to detect the expression of cell adhesion genes.Results·The SEM results of the laser treatment group showed no change in the surface morphology of the titanium sheet.There was no significant difference in the amount of cell adhesion and gene expression in each group of laser treatment on the surface of the titanium sheet(P>0.05),and laser treatment had no effect on it.Conclusion·Disinfection of implants by Nd:YAG water-cooled laser has no effect on the titanium implant surface and is safe for the treatment of peri-implantitis.

关 键 词：Nd:YAG水雾激光 种植体表面 安全性 

分 类 号：R781.4[医药卫生—口腔医学]