六价铬在斜带石斑鱼胚胎中的生物累积及其对igf2、glut2和pparg基因表达的影响  

EFFECTS OF WATERBORNE HEXAVALENT CHROMIUM(Cr6+)EXPOSURE ON CR BIOACCUMULATION,IGF2,GLUT2 AND PPARG EXPRESSION ON GROUPER EMBRYO DEVELOPMENT

作　　者：周小芳 张鹏飞 曾会玲 叶恒振 冯浩 李慧莹 靳方方 梁智策 郭志强 ZHOU Xiao-Fang;ZHANG Peng-Fei;ZENG Hui-Ling;YE Hen-Zhen;FENG Hao;LI Hui-Ying;JIN Fang-Fang;LIANG Zhi-Ceng;GUO Zhi-Qiang(State Key Laboratory of Marine Resource Utilization in South China Sea,School of Life and Pharmaceutical Sciences,College of Ocean,Hainan University,Haikou 570228,China;Key Laboratory of Tropical Biological Resource of Ministry of Education,Hainan University,Haikou 570228,China)

机构地区：[1]海南大学海洋学院,南海海洋资源利用国家重点实验室,海口570228 [2]海南大学生命科学与药学院,热带生物资源教育部重点实验室,海口570228

出　　处：《水生生物学报》2020年第6期1191-1198,共8页Acta Hydrobiologica Sinica

基　　金：国家自然科学基金(31872574和41866003);海南大学科研启动基金(KYQD(ZR)1803)资助。

摘　　要：以斜带石斑鱼(Epinephelus coioides)为研究对象,探讨了水中不同浓度梯度六价铬(0、0.20、0.60、1、5、10、20、40、60和80 mg/L)在胚胎发育不同阶段(桑葚期、囊胚期、原肠末期、脑泡形成期和心脏跳动期)的生物累积及其对细胞生长基因igf2和营养代谢基因glut2、pparg的mRNA表达的影响。结果表明,胚胎中Cr6+生物累积量和吸收率随暴露浓度的增加而增加,且吸收率随胚胎发育时期的延伸而降低。在桑葚期时,斜带石斑鱼胚胎吸收Cr6+能力最强,是心脏跳动期的20倍。在桑葚期、囊胚期和原肠末期,Cr6+对胚胎中igf2和glut2基因mRNA表达水平有显著促进作用(P<0.05),但对pparg基因mRNA表达水平有显著抑制作用(P<0.05)。在原肠末期和脑泡形成期,Cr6+对胚胎中pparg基因mRNA表达水平有显著促进作用(P<0.05)。研究表明水中Cr6+暴露在斜带石斑鱼胚胎发育过程中有明显的生物累积,且对细胞生长水平和营养代谢有明显的影响,研究为斜带石斑鱼早期生活史阶段适宜生境和资源保护提供了理论基础。To investigate the bioaccumulation and genotoxicity of waterborne hexavalent chromium(Cr6+)in the embryos of the grouper(Epinephelus coioides),the embryos were exposed to a gradient levels of hexavalent chromium(0,0.2,0.6,1,5,10,20,40,60 and 80 mg/L)for 30 hours to measure the Cr bioaccumulation and the mRNA expression levels of cell growth gene igf2,nutrient metabolism genes glut2 and pparg at the morula stage,the blastula stage,the late gastrula,the brain vesicle stage and the heart beating stage.The results showed that the Cr bioaccumulation and the uptake rate increased with the increased waterborne Cr6+,but the uptake rate decreased with the development of the embryo.The morula stage had the strongest ability to absorb waterborne Cr6+,which is 20 times that of the heart beating stage.At the morula stage,blastula stage and late gastrula,the waterborne Cr6+exposure significantly promoted igf2 and glut2 mRNA expression,while it clearly inhibited the pparg mRNA expression(P<0.05).At the late gastrula and the brain vesicle stage,the waterborne Cr6+exposure enhanced significantly pparg mRNA expression(P<0.05).These findings demonstrated that the waterborne Cr6+exposure had a significance effect on the Cr bioaccumulation,cell growth and nutrient metabolism of grouper embryos,which provide a theoretical basis for the grouper embryos protection in the aquatic environment.

关 键 词：六价铬 生物累积 斜带石斑鱼 胚胎 IGF2 GLUT2 PPARG 

分 类 号：S941.99[农业科学—水产养殖]