内源性大麻素N-花生四烯酸氨基乙醇介导卵巢癌细胞自噬和内质网应激  

Role of endoplasmic reticulum stress and autophagy mediated by endo⁃cannabinoid N-arachidonic acid aminoethanol in ovarian cancer cells

作　　者：黄静莹[1] 陈萱[1] 饶靖红 HUANG Jing-ying;CHEN Xuan;RAO Jing-hong(Department of Obstetrics and Gynecology,First Hospital of Quanzhou Affiliated to Fujian Medical University,Quanzhou 362000,China.)

机构地区：[1]福建医科大学附属泉州第一医院妇产科,福建泉州362000

出　　处：《中国病理生理杂志》2020年第11期2020-2029,共10页Chinese Journal of Pathophysiology

基　　金：福建省泉州市科技计划项目(No.2018Z073)。

摘　　要：目的:探讨内源性大麻素N-花生四烯酸氨基乙醇(AEA)在卵巢癌中的作用,并深入研究其作用机制。方法:ELISA实验分析健康对照组和卵巢癌组患者血清中AEA的水平,并结合ROC曲线评估AEA对卵巢癌患者的诊断价值;结合CCK-8实验、Transwell小室侵袭实验和划痕实验检测AEA对卵巢癌细胞活性、迁移和侵袭的影响;并通过肿瘤体积、瘤重的测量及瘤组织肉眼观图进一步验证AEA对卵巢癌的作用;同时用流式细胞术和Western blot检测AEA对卵巢癌细胞凋亡的影响,进而采用Western blot检测卵巢癌细胞自噬及内质网应激相关蛋白的水平,探究其促进卵巢癌细胞凋亡的机制。结果:卵巢癌患者体内AEA的含量较健康对照组显著下降,ROC结果提示AEA有望成为区分卵巢癌患者和健康个体的灵敏性生物学标志物,且AEA可以抑制卵巢癌细胞的活力、迁移、侵袭,抑制卵巢癌组织生长。结论:AEA通过促进卵巢癌细胞内质网应激并影响其自噬,进而促进卵巢癌细胞凋亡。AIM:To study the effect of endocannabinoid N-arachidonic acid aminoethanol(AEA)on ovarian cancer and its mechanism.METHODS:The serum levels of AEA in healthy control group and ovarian cancer group were analyzed by ELISA,and the diagnostic value of AEA in ovarian cancer patients was evaluated by ROC curve.The effects of AEA on the viability,migration and invasion abilities of the ovarian cancer cells were detected by CCK-8 assay,Tran⁃swell cell invasion test and Scratch test.The effect of AEA on ovarian cancer was further verified by the measurement of tu⁃mor volume,tumor weight and visual map of tumor tissue.Meanwhile,flow cytometry and Western blot were used to deter⁃mine the effect of AEA on the apoptosis of ovarian cancer cells,so as to explore the mechanism of AEA promoting the apop⁃tosis of ovarian cancer cells through detecting the endoplasmic reticulum stress and autophagy related proteins by Western blot.RESULTS:The serum levels of N-arachidonic aminoethanol in the patients with ovarian cancer were significantly decreased.ROC results suggested that AEA was a sensitive biological marker to distinguish the patients with ovarian can⁃cer from healthy dedividuals.In addition,AEA inhibited the cell viability,migration and invasion abilities of ovarian can⁃cer cells,and inhibited the growth of ovarian cancer tissues.CONCLUSION:By promoting endoplasmic reticulum stress and affecting autophagy of ovarian cancer cells,AEA promotes apoptosis of ovarian cancer cells.

关 键 词：内源性大麻素 N-花生四烯酸氨基乙醇 卵巢癌 自噬 内质网应激 

分 类 号：R737.32[医药卫生—肿瘤] R730.23[医药卫生—临床医学]