LncRNA-ATB在胃癌细胞中的表达及体外功能实验研究  

Expression of LncRNA-ATB in Gastric Cancer Cells and Its Function in Vitro

作　　者：智亮辉 刘伟[1] 李伟[1] 甄四虎 焦喜林 ZHI Liang-hui;LIU Wei;LI Wei;ZHEN Si-hu;JIAO Xi-lin(Department of General Surgery,the 980th Hospital of PLA Joint Logistics Support Forces,Shijiazhuang 050082,China)

机构地区：[1]中国人民解放军联勤保障部队第九八〇(白求恩国际和平)医院普通外科,石家庄050082

出　　处：《解放军医药杂志》2020年第11期16-20,44,共6页Medical & Pharmaceutical Journal of Chinese People’s Liberation Army

基　　金：河北省卫生健康委员会资助课题(20191215)。

摘　　要：目的研究LncRNA-ATB在胃癌细胞的表达及体外功能,为进一步筛选胃癌的有效诊治靶点奠定基础。方法设计LncRNA-ATB干扰序列,构建敲低LncRNA-ATB的胃癌细胞系并验证,进行克隆、增殖、迁移侵袭实验。结果LncRNA-ATB在胃癌细胞BGC-823、MGC-803中高表达,成功转染LncRNA-ATB干扰序列,并验证敲低效率,体外转染LncRNA-ATB干扰序列后,胃癌细胞克隆、增殖、迁移侵袭能力明显降低(P<0.05,P<0.01)。结论敲低LncRNA-ATB后胃癌细胞的克隆、增殖、迁移侵袭能力明显降低,表明其在胃癌进展中发挥重要的调控作用。Objective To study the expression of LncRNA-ATB in gastric cancer cells and its function in vitro,laying a foundation for further screening of effective diagnosis and treatment targets of gastric cancer.Methods The LncRNA-ATB interference sequence was designed to construct a knockdown lncRNA-ATB gastric cancer cell line,which was then verified by cloning,proliferation,migration and invasion experiments.Results LncRNA-ATB was highly expressed in gastric cancer cells BGC-823 and MGC-803.The LncRNA-ATB interference sequence was successfully transfected,and the knockdown efficiency was verified.After in vitro transfection of the LncRNA-ATB interference sequence,the cloning,proliferation,migration and invasion ability of gastric cancer cells was significantly reduced(P<0.05,P<0.01).Conclusion After knockdown of LncRNA-ATB,the ability of cloning,proliferation,migration and invasion of gastric cancer cells was significantly reduced,which indicated that LncRNA-ATB played an important regulatory role in the progression of gastric cancer.

关 键 词：胃肿瘤 胃癌细胞 lncRNA-ATB 细胞增殖 细胞迁徙 细胞克隆 侵袭 肿瘤 体外功能 

分 类 号：R735.2[医药卫生—肿瘤]