Gene transcription profiting of astheno-and normo-zoospermic sperm subpopulations  

作　　者：Pedro Caballero-Campo Saul Lira-Albarran David Barrera Elizabeth Borja-Cacho Hector S Godoy-Morales Claudia Rangel-Escareno Fernando Larrea Mayel Chirinos 

机构地区：[1]Department of Reproduction Biology,National Institute of Medical Sciences and Nutrition Salvador Zubiran,Mexico City 14080,Mexico [2]Tambre Foundation,Madrid 28002,Spain [3]Reproductive Medicine Unit,Angeles del Ped regal Hospital,Mexico City 10700,Mexico [4]Computational Genomic and Integrative Biology Laboratory,National Institute of Genomic Medicine,Mexico City 14610,Mexico

出　　处：《Asian Journal of Andrology》2020年第6期608-615,共8页亚洲男性学杂志（英文版）

摘　　要：Spermatozoa contain a repertoire of RNAs considered to be potential functional fertility biomarkers.In this study,the gene expression of human sperm subpopulations with high(F1)and low(F2)motility from healthy normozoospermic(N)and asthenozoospermic(A)individuals was evaluated using RNA microarray followed by functional genomic analysis of differentially expressed genes.Results from A-F1 versus N-F1,A-F2 versus N-F2,N-F1 versus N-F2,and A-F1 versus A-F2 comparisons showed a considerably larger set of downregulated genes in tests versus controls.Gene ontology(GO)analysis of A-F1 versus N-F1 identified 507 overrepresented biological processes(BPs),several of which are associated with sperm physiology.In addition,gene set enrichment analysis of the same contrast showed 110 BPs,36 cellular comp on ents,and 31 molecular functions,several of which are involved in sperm motility.A leadi ng・edge analysis of selected GO terms resulted in several down regulated genes encoding to dyn eins and kin esins,both related to sperm physiology.Furthermore,the predicted activation state of asthenozoospermia was increased,while fertility,cell movement of sperm,and gametogenesis were decreased.Interestingly,several downregulated genes characteristic of the canonical pathway protein ubiquitination were involved in asthenozoospermia activation.Conversely,GO analysis of A-F2 versus N-F2 did not identify overrepresented BPs,although the gene set enrichment analysis detected six enriched BPs,one cellular component,and two molecular functions.Overall,the results show differences in gene transcription between sperm subpopulations from asthenozoospermic and normozoospermic semen samples and allowed the identification of gene sets relevant to sperm physiology and reproduction.

关 键 词：ASTHENOZOOSPERMIA male in fertility MICROARRAY SPERM TRANSCRIPTOME 

分 类 号：R698.2[医药卫生—泌尿科学]