酿酒酵母关联多药耐受性转录因子Yrr1p的研究进展  

Research Advances on Transcription Factor Yrr1p of Pleiotropic Drug Resistance in Saccharomyces cerevisiae

作　　者：曹文妍 王心凝 沈煜 李在禄 鲍晓明 CAO Wen-yan;WANG Xin-ning;SHEN Yu;LI Zai-lu;BAO Xiao-ming(State Key Laboratory of Bio-based Material and Green Papermaking,Shandong Provincial Key Laboratory of Microbial Engineering,School of Bioengineering,Jinan 250353;State Key Laboratory of Microbial Technology,Microbiology Technology Institute,Shandong University,Qingdao 266237)

机构地区：[1]齐鲁工业大学生物工程学院生物基材料与绿色造纸国家重点实验室山东省微生物工程重点实验室,济南250353 [2]山东大学微生物技术研究院微生物技术国家重点实验室,青岛266237

出　　处：《生物技术通报》2020年第11期148-154,共7页Biotechnology Bulletin

基　　金：国家重点研发计划(2018YFB1501401);国家自然科学基金面上项目(31870063);山东省自然科学基金博士基金(ZR2019BC080);山东省自然科学基金重大基础研究项目(ZR2018ZB0209)。

摘　　要：Yrr1p(for yeast Reveromycin A resistance)是含有锌指结构、关联多药耐受性的转录因子,在介导酿酒酵母耐药及抑制物抗性方面具有重要作用。本文回顾了Yrr1p的发现过程,综述了Yrr1p的结构与功能的关系及其介导酿酒酵母对4-硝基喹啉-N-氧化物、水杨酸、戴森锰锌及香草醛等抑制物的耐受机制。其中,Yrr1p介导香草醛耐受性机制与其他药物不同。并概括了Yrr1p与其同源转录因子Yrm1p、Pdr8p,以及上级调控因子Pdr1p、Pdr3p之间的调控关系。转录因子Yrr1p的研究对微生物耐药性研究以及构建高抗性微生物细胞工厂具指导意义。Yrr1p(for yeast reveromycin A resistance)is a zinc finger transcription factor involved in the pleiotropic drug resistance(PDR),and plays important role in the drug resistance and inhibitor resistance of Saccharomyces cerevisiae.This paper reviewed the discovery process of Yrr1p,the relationship between Yrr1p structure and function,and the mechanism of resistances mediated by Yrr1p in S.cerevisiae to different inhibitors of 4-nitroquinoline-N-oxide,salicylic acid,mancozeb,and vanillin.Tolerance mechanism to vanillin stress mediated by Yrr1p displayed remarkable differences from other drugs.Further the paper outlined the regulatory relationship between Yrr1p and its homologous transcription factors Yrm1p,Pdr8p,and its upstream regulatory factors Pdr1p,Pdr3p.The research of Yrr1p provides scientific guidance for studying microbial tolerance and constructing highly resistant microbial cell factories.

关 键 词：Yrr1p 转录因子 耐受性 酿酒酵母 

分 类 号：Q78[生物学—分子生物学]